# denotes equal co-authorship; *denotes co-corresponding authorship
2026
Fong P-M, Weaver AI, Manson AL, Park Y, Earl AM, Walker S*. Staphylococcus aureus uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis. bioRxiv. 2026. 2026.06.23.733835. [Publisher Link]
Russell BJ, Hasenoehrl E, Marando VM, Lu J, Chen JM, James MJ, Goyal M, Walker S, Rakoff-Nahoum S, Jost M*. Identification of the complete pathway for conversion of bilirubin to urobilinogen by human gut bacteria. bioRxiv 2026. 2026.06.10.731317. [PubMed Link] [Publisher Link]
Tükenmez H, Nye TM, Singh P, Mychack A, Bonde M, Hickerson S, Obernuefemann CLP, Pinkner JS, Zhu S, Sarkar S, Bharate JB, van der Lingen I, Ntuyenm AQ, Rao VUB, Lindgren AEG, Klein H, Zou Z, Dodson KW, Walker S, Mateus A, Johansson J, Caparon MG, Almqvist F, Hultgren SJ*. Tunable TriPcides suppress virulence factor secretion during Staphylococcus aureus infection and kill dormant cells. Sci Adv. 2026. 8, 12(19):eaec9100. [PubMed Link] [Publisher Link]
Ostash B, Makitrynskyy R, Fedchyshyn M, Luzhetskyy A, Walker S, Fedorenko V*. Genetically engineered Streptomyces viridosporus ATCC 14672 strains for the discovery of novel moenomycins. Sci Rep. 2026.10, 16(1):12851. [PubMed Link] [Publisher Link]
Scarpa de Mello S, Schultz B, Goh B, Grote A, Shea T, Muscato J, Oh SF, Manson AL, Earl AM, Walker S, Camargo ILBC, Gilmore MS*. Loss of LafB activity reverses daptomycin resistance in E. faecium. mBio. 2026. 14, 17(1):e0071525. [PubMed Link] [Publisher Link]
2025
Hart EM, de Bruin DA, Marando VM, Alemany MA, Snow ED, Schultz BJ, Kuru E, Walker S, Vettiger A, Bernhardt TG*. The mycomembrane proteins PorH and ProtX are inserted at polar growth zones and linked to the cell wall. bioRxiv. 2025. 14: 025.10.14.682376. [PubMed Link] [Publisher Link]
Sisley, T.A.; #Park , Y.; #Santiago, A.G.; Beroual, W.; Sobolewski, I.A.; Lee, W.; Paulo, J.A.; *Walker, S. AuxB interacts directly with GpsB and PknB to coordinate cell envelope processes that contribute to intrinsic antibiotic resistance in Staphylococcus aureus. mBio. 2025. 16(10):e0185825. [PubMed Link] [Publisher Link]
Chang, JO.; Lee, CY.; Kim, I.; Kim, J.; Kim, JH.; Yun, T,; Hooper, D.C.; *Walker, S.; *Lee, W.; Environmental cues in different host niches shape the survival fitness of Staphylococcus aureus. Nature Commun. 2025. 16, 6928. [PubMed Link] [Publisher Link]
#Park, Y.; #Sisley, T.A.; #Stone, M.C.; Corrigan, R.M.; Vickery, C.R.; Gründling, A.; *Walker, S.; SpbR controls lipoteichoic acid length by directly inhibiting signal peptidase SpsB in Staphylococcus aureus. Proc. Natl. Acad. Sci. 2025. 122 (27), e2426464122. [PubMed Link] [Publishers Link]
Anderson, A.C.; #Schultz, B.J.; #Snow, E.D.; Brott, A.S.; Stangherlin, S.; Malloch, T.; London, J.R.; Walker, S.; *Clarke, A.J.; The mechanism of peptidoglycan O-acetylation in Gram-negative bacteria typifies bacterial MBOAT-SGNH acyltransferases. J. Biol. Chem. 2025. 301 (6), 108531. [PubMed Link] [Publishers Link]
Filsinger, G.T.; Mychack, A.; Lyerly, E.; Henriksen, C.; Bartlett, T.M.; Kuchwara, H.; Eitzinger, S.; Bernhardt, T.G.; Walker, S.; *Church, G.M.; *Wannier, T.M.; A diverse single-stranded DNA-annealing protein library enables efficient genome editing across bacterial phyla. Proc. Natl. Acad. Sci. 2025. 22 (17), e2414342122. [PubMed Link] [Publishers Link]
Schultz, B.J.; *Walker, S.; Acyltransferases that Modify Cell Surface Polymers Across the Membrane. Biochemistry. 2025. 64 (8), 1728-1749. [PubMed Link] [Publishers Link]
Mychack, A.; Evans, D.; Gilles, T.; James, M.J.; *Walker, S.; Staphylococcus aureus uses a GGDEF protein to recruit diacylglycerol kinase to the membrane for lipid recycling. Proc. Natl. Acad.Sci. 2025. 122 (12), e2414696122. [PubMed Link] [Publishers Link]
2024
Anderson, A. C.; #Schultz, B. J.; #Snow, E. D.; Brott, A. S.; Stangherlin, S.; Malloch, T.; London, J. R.; Walker, S.; *Clarke, A. J. The mechanism of peptidoglycan O-acetylation in Gram-negative bacteria typifies bacterial MBOAT-SGNH acyltransferases. bioRxiv. 2024. [PubMed Link] [PubMed Link]
Hammel, F. A.; Payne, N. C.; Marando, V. M.; Mazitschek, R.; *Walker, S. Identification of a Polypeptide Inhibitor of O-GlcNAc Transferase with Picomolar Affinity. J. Am. Chem. Soc. 2024. 146, 26320-26330. [PubMed Link] [Publisher Link]
Potter, S. C.; Gibbs, B. E.; Hammel, F. A.; Joiner, C. M.; Paulo, J. A.; Janetzko, J.; Levine, Z. G.; Fei, G. Q.; Haggarty, S. J.; *Walker, S. Dissecting OGT’s TPR domain to identify determinants of cellular function. Proc. Natl. Acad. Sci. U. S. A. 2024, 121 (22), e2401729121. [PubMed Link] [Publisher Link]
Bartlett, T. M.; Sisley, T. A.; Mychack, A.; Walker, S.; Baker, R. W.; *Rudner, D. Z.; *Bernhardt, T. G. FacZ is a GpsB-interacting protein that prevents aberrant division-site placement in Staphylococcus aureus. Nat. Microbiol. 2024, 9, 801-813. [PubMed Link] [Publisher Link]
Beetham, C. M.; Schuster, C. F.; Kviatkovski, I.; Santiago, M.; Walker, S.; *Gründling, A. Histidine transport is essential for the growth of Staphylococcus aureus at low pH. PLoS Pathog. 2024, 20 (1), e1011927. [PubMed Link] [Publisher Link]
2023
Schultz, B. J.; Snow, E. D.; *Walker, S. Mechanism of ᴅ-alanine transfer to teichoic acids shows how bacteria acylate cell envelope polymers. Nat. Microbiol. 2023, 8 (7), 1318-1329. [PubMed Link] [Publisher Link]
Shlosman, I.; Fivenson, E. M.; Gilman, M. S. A.; Sisley, T. A.; Walker, S.; Bernhardt, T. G.; *Kruse, A. C.; *Loparo, J. J. Allosteric activation of cell wall synthesis during bacterial growth. Nat. Commun. 2023, 14 (1), 3439. [PubMed Link] [Publisher Link]
#Park, Y.; #Taguchi, A.; Baidin, V.; Kahne, D.; *Walker, S. A Time-Resolved FRET Assay Identifies a Small Molecule that Inhibits the Essential Bacterial Cell Wall Polymerase FtsW. Angew. Chem. Int. Ed. 2023, e202301522. [PubMed Link] [Publisher Link]
Stone, M. C.; Mychack, A.; Coe, K. A.; *Walker, S. Combining Signal Peptidase and Lipoprotein Processing Inhibitors Overcomes Ayr Resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 2023, e00115-23. [PubMed Link] [Publisher Link]
Hummels, K. R.; Berry, S. P.; Li, Z.; Taguchi, A.; Min, J. K.; Walker, S.; Marks, D. S.; *Bernhardt, T. G. Coordination of bacterial cell wall and outer membrane biosynthesis. Nature 2023, 615 (7951), 300-304. [PubMed Link] [Publisher Link]
2022
Page, J. E.; Skiba, M. A.; Do, T.; Kruse, A. C.; *Walker, S. Metal cofactor stabilization by a partner protein is a widespread strategy employed for amidase activation. Proc. Natl. Acad. Sci. U. S. A. 2022, 119 (26), e2201141119. [PubMed Link] [Publisher Link]
Gondane, A.; Poulose, N.; Walker, S.; Mills, I. G.; *Itkonen, H. M. O-GlcNAc transferase maintains metabolic homeostasis in response to CDK9 inhibition. Glycobiology 2022, 00, 1-9. [PubMed Link] [Publisher Link]
#Muscato, J. D.; #Morris, H. G.; Mychack, A.; Rajagopal, M.; Baidin, V.; Hesser, A.; Lee, W.; İnecik, K.; Wilson, L. J.; Kraml, C. M.; Meredith, T. C.; *Walker, S. Rapid inhibitor discovery by exploiting synthetic lethality. J. Am. Chem. Soc. 2022, 144 (8), 3696-3705. [PubMed Link] [Publisher Link]
2021
Taguchi, A.; Page, J. E.; Tsui, H.-C. T.; Winkler, M. E.; *Walker, S. Biochemical reconstitution defines new functions for membrane-bound glycosidases in assembly of the bacterial cell wall. Proc. Natl. Acad. Sci. U. S. A. 2021, 118 (36), e2103740118. [PubMed Link] [Publisher Link]
*Joiner, C. M.; Hammel, F. A.; Janetzko, J.; *Walker, S. Protein Substrates Engage the Lumen of O-GlcNAc Transferase’s Tetratricopeptide Repeat Domain in Different Ways. Biochemistry 2021, 60 (11), 847-853. [PubMed Link] [Publisher Link]
Page, J. E.; *Walker, S. Natural products that target the cell envelope. Curr. Opin. Microbiol. 2021, 61, 16-24. [PubMed Link] [Publisher Link]
Levine, Z. G.; Potter, S. C.; Joiner, C. M.; Fei, G. Q.; Nabet, B.; Sonnett, M.; Zachara, N. E.; Gray, N. S.; Paulo, J. A.; *Walker, S. Mammalian cell proliferation requires noncatalytic functions of O-GlcNAc transferase. Proc. Natl. Acad. Sci. U. S. A. 2021, 118 (4), e2016778118. [PubMed Link] [Publisher Link]
#Schaefer, K.; #Owens, T. W.; Page, J. E.; Santiago, M.; Kahne, D.; *Walker, S. Structure and reconstitution of a hydrolase complex that may release peptidoglycan from the membrane after polymerization. Nat. Microbiol. 2021, 6, 34-43. [PubMed Link] [Publisher Link]
2020
Hesser, A. R.; Schaefer, K.; Lee, W.; Walker, S. Lipoteichoic acid polymer length is determined by competition between free starter units. Proc. Natl. Acad. Sci. U. S. A. 2020, 117 (47), 29669-29676. [PubMed Link] [Publisher Link]
Itkonen, H. M.; Poulose, N.; Steele, R. E.; Martin, S. E. S.; Levine, Z. G.; Duveau, D. Y.; Carelli, R.; Singh, R.; Urbanucci, A.; Loda, M.; Thomas, C. J.; Mills, I. G.; Walker, S. Inhibition of O-GlcNAc Transferase Renders Prostate Cancer Cells Dependent on CDK9. Mol. Cancer Res. 2020, 18 (10), 1512-1521. [PubMed Link] [Publisher Link]
#Hesser, A. R.; #Matano, L. M.; #Vickery, C. R.; Wood, B. M.; Santiago, A. G.; Morris, H. G.; Do, T.; Losick, R.; Walker, S. The Length of Lipoteichoic Acid Polymers Controls Staphylococcus aureus Cell Size and Envelope Integrity. J. Bacteriol. 2020, 202 (16), e00149-20. [PubMed Link] [Publisher Link]
#Tan, Z.-W.; #Fei, G.; Paulo, J. A.; Bellaousov, S.; Martin, S. E. S.; Duveau, D. Y.; Thomas, C. J.; Gygi, S. P.; *Boutz, P. L.; *Walker, S. O-GlcNAc regulates gene expression by controlling detained intron splicing. Nucleic Acids Res. 2020, 48 (10), 5656-5669. [PubMed Link] [Publisher Link]
Laut, C. L.; Perry, W. J.; Metzger, A. L.; Weiss, A.; Stauff, D. L.; Walker, S.; Caprioli, R. M.; Skaar, E. P. Bacillus anthracis Responds to Targocil-Induced Envelope Damage through EdsRS Activation of Cardiolipin Synthesis. mBio 2020, 11 (2), e03375-19. [PubMed Link] [Publisher Link]
Sjodt, M.; Rohs, P. D. A.; Gilman, M. S. A.; Erlandson, S. C.; Zheng, S.; Green, A. G.; Brock, K. P.; Taguchi, A.; Kahne, D.; Walker, S.; Marks, D. S.; Rudner, D. Z.; Bernhardt, T. G.; Kruse, A. C. Structural coordination of polymerization and crosslinking by a SEDS-bPBP peptidoglycan synthase complex. Nat. Microbiol. 2020, 5 (6), 813-820. [PubMed Link] [Publisher Link]
#Rubino, F. A.; #Mollo, A.; Kumar, S.; Butler, E. K.; *Ruiz, N.; *Walker, S.; *Kahne, D. E. Direction of Transport Intermediates in the Peptidoglycan Flippase MurJ Identifies Residues Essential for Conformational Cycling. J. Am. Chem. Soc. 2020, 142 (12), 5482-5486. [PubMed Link] [Publisher Link]
Do, T.; Page, J. E.; Walker, S. Uncovering the activities, biological roles, and regulation of bacterial cell wall hydrolases and tailoring enzymes. J. Biol. Chem. 2020, 295 (10), 3347-3361. [PubMed Link] [Publisher Link]
Palace, S. G.; Wang, Y.; Rubin, D. H. F.; Welsh, M. A.; Mortimer, T. D.; Cole, K.; Eyre, D. W.; Walker, S.; Grad, Y. H. RNA polymerase mutations cause cephalosporin resistance in clinical Neisseria gonorrhoeae isolates. eLife 2020, 9, e51407. [PubMed Link] [Publisher Link]
Do, T.; Schaefer, K.; Santiago, A. G.; Coe, K. A.; Fernandes, P. B.; Kahne, D.; Pinho, M. G.; Walker, S. Staphylococcus aureus cell growth and division are regulated by an amidase that trims peptides from uncrosslinked peptidoglycan. Nat. Microbiol. 2020, 5 (2), 291-303. [PubMed Link] [Publisher Link]
2019
Schuster, C. F.; Wiedemann, D. M.; Kirsebom, F. C. M.; Santiago, M.; Walker, S.; Gründling, A. High-throughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistant Staphylococcus aureus and underlies a tailored response to different osmotic stressors. Mol. Microbiol. 2019, xx (xx), x-x. [PubMed Link] [Publisher Link]
#Coe, K. A.; #Lee, W.; Stone, M. C.; Komazin-Meredith, G.; *Meredith, T. C.; *Grad, Y. H.; *Walker, S. Multi-strain Tn-Seq reveals common daptomycin resistance determinants in Staphylococcus aureus. PLoS Pathog. 2019, 15 (11), e1007862. [PubMed Link] [Publisher Link]
#Joiner, C. M.; #Levine, Z. G.; Aonbangkhen, C.; Woo, C. M.; Walker, S. Aspartate Residues Far from the Active Site Drive O-GlcNAc Transferase Substrate Selection. J. Am. Chem. Soc. 2019, 141 (33), 12974-12978. [PubMed Link] [Publisher Link]
Taguchi, A.; Kahne, D.; Walker, S. Chemical tools to characterize peptidoglycan synthases. Curr. Opin. Chem. Biol. 2019, 53, 44-50. [PubMed Link] [Publisher Link]
#Welsh, M. A.; #Schaefer, K.; Taguchi, A.; Kahne, D.; Walker, S. Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases. J. Am. Chem. Soc. 2019, 141 (33), 12994-12997. [PubMed Link] [Publisher Link]
#Lane, E. A.; #Choi, D. W.; Garcia-Haro, L.; Levine, Z. G.; Tedoldi, M.; Walker, S.; Danial, N. N. HCF-1 Regulates De Novo Lipogenesis through a Nutrient-Sensitive Complex with ChREBP. Mol. Cell 2019, 25 (2), 357-371.e7. [PubMed Link] [Publisher Link]
Itkonen, H. M.; Poulose, N.; Walker, S.; Mills, I. G. CDK9 Inhibition Induces a Metabolic Switch that Renders Prostate Cancer Cells Dependent on Fatty Acid Oxidation. Neoplasia 2019, 21 (7), 713-720. [PubMed Link] [Publisher Link]
Itkonen, H. M.; Urbanucci, A.; Martin, S. E. S.; Khan, A.; Mathelier, A.; Thiede, B.; Walker, S.; Mills, I. G. High OGT activity is essential for MYC-driven proliferation of prostate cancer cells. Theranostics 2019, 9 (8), 2183-2197. [PubMed Link] [Publisher Link]
Taguchi, A.; Welsh, M. A.; Marmont, L. S.; Lee, W.; Sjodt, M.; Kruse, A. C.; Kahne, D.; *Bernhardt, T. G.; *Walker, S. FtsW is a peptidoglycan polymerase that is functional only in complex with its cognate penicillin-binding protein. Nat. Microbiol. 2019, 4, 587-594. [PubMed Link] [Publisher Link]
Joiner, C. M.; Li, H.; Jiang, J.; Walker, S. Structural characterization of the O-GlcNAc cycling enzymes: insights into substrate recognition and catalytic mechanisms. Curr. Opin. Struct. Biol. 2019, 56, 97-106. [PubMed Link] [Publisher Link]
Sehin, Y.; Koshla, O.; Dacyuk, Y.; Zhao, R.; Ross, R.; Myronovskyi, M.; Limbach, P. A.; Luzhetskyy, A.; Walker, S.; Fedorenko, V.; Ostash, B. Gene ssfg_01967 (miaB) for tRNA modification influences morphogenesis and moenomycin biosynthesis in Streptomyces ghanaensis ATCC14672. Microbiology 2019, 165 (2), 233-245. [PubMed Link] [Publisher Link]
2018
Wood, B. M.; Santa Maria, J. P.; Matano, L. M.; Vickery, C. R.; Walker, S. A partial reconstitution implicates DltD in catalyzing lipoteichoic acid D-alanylation. J. Biol. Chem. 2018, 293 (46), 17985-17996. [PubMed Link] [Publisher Link]
#Martin, S. E. S.; #Tan, Z.-W.; Itkonen, H. M.; Duveau, D. Y.; Paulo, J. A.; Janetzko, J.; Boutz, P. L.; Törk, L.; Moss, F. A.; Thomas, C. J.; Gygi, S. P.; Lazarus, M. B.; Walker, S. Structure-Based Evolution of Low Nanomolar O-GlcNAc Transferase Inhibitors. J. Am. Chem. Soc. 2018, 140 (42), 13542-13545. [PubMed Link] [Publisher Link]
Rohs, P. D. A.; Buss, J.; Sim, S. I.; Squyres, G. R.; Srisuknimit, V.; Smith, M.; Cho, H.; Sjodt, M.; Kruse, A. C.; Garner, E. C.; Walker, S.; Kahne, D. E.; Bernhardt, T. G. A central role for PBP2 in the activation of peptidoglycan polymerization by the bacterial cell elongation machinery. PLoS Genet. 2018, 14 (10), e1007726. [PubMed Link] [Publisher Link]
Baranowski, C.; Welsh, M. A.; Sham, L.-T.; Eskandarian, H. A.; Lim, H. C.; Kieser, K. J.; Wagner, J. C.; McKinney, J. D.; Fantner, G. E.; Ioerger, T. R.; Walker, S.; Bernhardt, T. G.; Rubin, E. J.; Rego, E. H. Maturing Mycobacterium smegmatis peptidoglycan reüluires non-canonical crosslinks to maintain shape. eLife 2018, 16 (7), e37516. [PubMed Link] [Publisher Link]
Buss, J. A.; Baidin, V.; Welsh, M. A.; Flores-Kim, J.; Cho, H.; Wood, B. M.; Uehara, T.; Walker, S.; Kahne, D. Bernhardt, T. G. Pathway-Directed Screen for Inhibitors of the Bacterial Cell Elongation Machinery. Antimicrob. Agents Chemother. 2018, 63 (1), e01530-18. [PubMed Link] [Publisher Link]
Lee, W.; Do, T.; Zhang, G.; Kahne, D.; Meredith, T. C.; Walker, S. Antibiotic Combinations That Enable One-Step, Targeted Mutagenesis of Chromosomal Genes. ACS Infect. Dis. 2018, 4 (6), 1007-1018. [PubMed Link] [Publisher Link]
Rubino, F. A.; Kumar, S.; Ruiz, N.; *Walker, S.; *Kahne, D. E. Membrane Potential Is Required for MurJ Function. J. Am. Chem. Soc. 2018, 140 (13), 4481-4484. [PubMed Link] [Publisher Link]
Sjodt, M.; Brock, K.; Dobihal, G.; Rohs, P. D. A.; Green, A. G.; Hopf, T. A.; Meeske, A. J.; Srisuknimit, V.; Kahne, D.; Walker, S.; Marks, D. S.; Bernhardt, T. G.; Rudner, D. Z.; Kruse, A. C. Structure of the peptidoglycan polymerase RodA resolved by evolutionary coupling analysis. Nature 2018, 556 (7699), 118-121. [PubMed Link] [Publisher Link]
Tiwari, K. B.; Gatto, C.; Walker, S.; Wilkinson, B. J. Exposure of Staphylococcus aureus to Targocil Blocks Translocation of the Major Autolysin Atl across the Membrane, Resulting in a Significant Decrease in Autolysis. Antimicrob. Agents Chemother. 2018, 62 (7), e00323-18. [PubMed Link] [Publisher Link]
#Santiago, M.; #Lee, W.; Fayad, A. A.; Coe, K. A.; Rajagopal, M.; Do, T.; Hennessen, F.; Srisuknimit, V.; Müller, R.; Meredith, T. C.; Walker, S. Genome-wide mutant profiling predicts the mechanism of a Lipid II binding antibiotic. Nat. Chem. Biol. 2018, 14 (6), 601-608. [PubMed Link] [Publisher Link]
Levine, Z. G.; Fan, C.; Melicher, M. S.; Orman, M.; Benjamin, T.; Walker, S. O-GlcNAc Transferase Recognizes Protein Substrates Using an Asparagine Ladder in the Tetratricopeptide Repeat (TPR) Superhelix. J. Am. Chem. Soc. 2018, 140 (10), 3510-3513. [PubMed Link] [Publisher Link]
#Hussain, S.; #Wivagg, C. N.; Szwedziak, P.; Wong, F.; Schaefer, K.; Izoré, T.; Renner, L. D.; Holmes, M. J.; Sun, Y.; Bisson-Filho, A. W.; Walker, S.; Amir, A.; Löwe, J.; Garner, E. C. MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis. eLife 2018, 22 (7), e32471. [PubMed Link] [Publisher Link]
#Schaefer, K.; #Owens, T. W.; Kahne, D.; Walker, S. Substrate Preferences Establish the Order of Cell Wall Assembly in Staphylococcus aureus. J. Am. Chem. Soc. 2018, 140 (7), 2442-2445. [PubMed Link] [Publisher Link]
Vickery, C. R.; Wood, B. M.; Morris, H. G.; Losick, R.; Walker, S. Reconstitution of Staphylococcus aureus Lipoteichoic Acid Synthase Activity Identifies Congo Red as a Selective Inhibitor. J. Am. Chem. Soc. 2018, 140 (3), 876-879. [PubMed Link] [Publisher Link]
2017
Welsh, M. A.; Taguchi, A.; Schaefer, K.; Van Tyne, D.; Lebreton, F.; Gilmore, M. S.; Kahne, D.; Walker, S. Identification of a Functionally Unique Family of Penicillin-Binding Proteins. J. Am. Chem. Soc. 2017, 139 (49), 17727-17730. [PubMed Link] [Publisher Link]
#Matano, L. M.; #Morris, H. G.; #Hesser, A. R.; Martin, S. E. S.; Lee, W.; Owens, T. W.; Laney, E.; Nakaminami, H.; Hooper, D.; Meredith, T. C.; Walker, S. Antibiotic That Inhibits the ATPase Activity of an ATP-Binding Cassette Transporter by Binding to a Remote Extracellular Site. J. Am. Chem. Soc. 2017, 139 (31), 10597-10600. [PubMed Link] [Publisher Link]
#DeFrancesco, A. S.; #Masloboeva, N.; Syed, A. K.; DeLoughery, A.; Bradshaw, N.; Li, G.-W.; Gilmore, M. S.; Walker, S.; Losick, R. Genome-wide screen for genes involved in eDNA release during biofilm formation by Staphylococcus aureus. Proc. Natl. Acad. Sci. U. S. A. 2017, 114 (29), E5969-E5978. [PubMed Link] [Publisher Link]
#Qiao, Y.; #Srisuknimit, V.; Rubino, F.; Schaefer, K.; Ruiz, N.; Walker, S.; Kahne, D. Lipid II overproduction allows direct assay of transpeptidase i, nhibition by β-lactams. Nat. Chem. Biol. 2017, 13 (7), 793-798. [PubMed Link] [Publisher Link]
#Srisuknimit, V.; #Qiao, Y.; Schaefer, K.; Kahne, D.; Walker, S. Peptidoglycan Cross-Linking Preferences of Staphylococcus aureus Penicillin-Binding Proteins Have Implications for Treating MRSA Infections. J. Am. Chem. Soc. 2017, 139 (29), 9791-9794. [PubMed Link] [Publisher Link]
Schaefer, K.; Matano, L. M.; Qiao, Y.; Kahne, D.; Walker, S. In vitro reconstitution demonstrates the cell wall ligase activity of LCP proteins. Nat. Chem. Biol. 2017, 13 (4), 396-401. [PubMed Link] [Publisher Link]
Janetzko, J.; Walker, S. Aspartate Glycosylation Triggers Isomerization to Isoaspartate. J. Am. Chem. Soc. 2017, 139 (9), 3332-3335. [PubMed Link] [Publisher Link]
Lee, W.; VanderVen, B. C.; Walker, S.; Russell, D. G. Novel protein acetyltransferase, Rv2170, modulates carbon and energy metabolism in Mycobacterium tuberculosis. Sci. Rep. 2017, 7 (1), 72. [PubMed Link] [Publisher Link]
Rajagopal, M.; Walker, S. Envelope Structures of Gram-Positive Bacteria. Curr. Top. Microbiol. Immunol. 2017, 404, 1-44. [PubMed Link] [Publisher Link]
2016
Matano, L. M.; Morris, H. G.; Wood, B. M.; Meredith, T. C.; Walker, S. Accelerating the discovery of antibacterial compounds using pathway-directed whole cell screening. Bioorg. Med. Chem. 2016, 24 (24), 6307-6314. [PubMed Link] [Publisher Link]
Janetzko, J.; Trauger, S. A.; Lazarus, M. B.; Walker, S. How the glycosyltransferase OGT catalyzes amide bond cleavage. Nat. Chem. Biol. 2016, 12 (11), 899-901. [PubMed Link] [Publisher Link]
#Rajagopal, M.; #Martin, M. J.; #Santiago, M.; Lee, W.; Kos, V. N.; Meredith, T.; Gilmore, M. S.; Walker, S. Multidrug Instrinsic Resistance Factors in Staphylococcus aureus Identified by Profiling Fitness within High-Diversity Transposon Libraries. mBio 2016, 7 (4), e00950-16. [PubMed Link] [Publisher Link]
Meeske, A. J.; Riley, E. P.; Robins, W. P.; Uehara, T.; Mekalanos, J. J.; Kahne, D.; Walker, S.; Kruse, A. C.; Bernhardt, T. G.; Rudner, D. Z. SEDS proteins are a widespread family of bacterial cell wall polymerases. Nature 2016, 537 (7622), 634-638. [PubMed Link] [Publisher Link]
Levine, Z. G.; Walker, S. The Biochemistry of O-GlcNAc Transferase: Which Functions Make It Essential in Mammalian Cells? Annu. Rev. Biochem. 2016, 85, 631-657. [PubMed Link] [Publisher Link]
#Horbal, L.; #Ostash, B.; Luzhetskyy, A.; Walker, S.; Kalinowski, J.; Fedorenko, V. A gene cluster for the biosynthesis of moenomycin family antibiotics in the genome of teicoplanin producer Actinoplanes teichomyceticus. Appl. Microbiol. Biotechnol. 2016, 100 (17), 7629-7638. [PubMed Link] [Publisher Link]
Markovski, M.; Bohrhunter, J. L.; Lupoli, T. J.; Uehara, T.; Walker, S.; Kahne, D. E.; Bernhardt, T. G. Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity. Proc. Natl. Acad. Sci. U. S. A. 2016, 113 (17), 4788-4793. [PubMed Link] [Publisher Link]
Goodreid, J. D.; Janetzko, J.; Santa Maria, J. P.; Wong, K. S.; Leung, E.; Eger, B. T.; Bryson, S.; Pai, E. F.; Gray-Owen, S. D.; Walker, S.; Houry, W. A.; Batey, R. A. Development and Characterization of Potent Cyclic Acyldepsipeptide Analogues with Increased Antimicrobial Activity. J. Med. Chem. 2016, 59 (2), 624-646. [PubMed Link] [Publisher Link]
Lee, W.; Schaefer, K.; Qiao, Y.; Srisuknimit, V.; Steinmetz, H.; Müller, R.; Kahne, D.; Walker, S. The Mechanism of Action of Lysobactin. J. Am. Chem. Soc. 2016, 138 (1), 100-103. [PubMed Link] [Publisher Link]
Pasquina, L.; Santa Maria, J. P.; Wood, B. M.; Moussa, S. H.; Matano, L. M.; Santiago, M.; Martin, S. E. S.; Lee, W.; Meredith, T. C.; Walker, S. A synthetic lethal approach for compound and target identification in Staphylococcus aureus. Nat. Chem. Biol. 2016, 12 (1), 40-45. [PubMed Link] [Publisher Link]
2015
Angelova, M.; Ortiz-Meoz, R. F.; Walker, S.; Knipe, D. M. Inhibition of O-Linked N-Acetylglucosamine Transferase Reduces Replication of Herpes Simplex Virus and Human Cytomegalovirus. J. Virol. 2015, 89 (16), 8474-8483. [PubMed Link] [Publisher Link]
Santiago, M.; Matano, L. M.; Moussa, S. H.; Gilmore, M. S.; Walker, S.; Meredith, T. C. A new platform for ultra-high density Staphylococcus aureus transposon libraries. BMC Genomics 2015, 16, 252. [PubMed Link] [Publisher Link]
Ortiz-Meoz, R. F.; Jiang, J.; Lazarus, M. B.; Orman, M.; Janetzko, J.; Fan, C.; Duveau, D. Y.; Tan, Z.-W.; Thomas, C. J.; Walker, S. A Small Molecule That Inhibits OGT Activity in Cells. ACS Chem. Biol. 2015, 10 (6), 1392-1397. [PubMed Link] [Publisher Link]
Schirner, K.; Eun, Y.-J.; Dion, M.; Luo, Y.; Helmann, J. D.; Garner, E. C.; Walker, S. Lipid-linked cell wall precursors regulate membrane association of bacterial actin MreB. Nat. Chem. Biol. 2015, 11 (1), 38-45. [PubMed Link] [Publisher Link]
2014
Qiao, Y.; Lebar, M. D.; Schirner, K.; Schaefer, K.; Tsukamoto, H.; Kahne, D.; Walker, S. Detection of Lipid-Linked Peptidoglycan Precursors by Exploiting an Unexpected Transpeptidase Reaction. J. Am. Chem. Soc. 2014, 136 (42), 14678-14681. [PubMed Link] [Publisher Link]
Janetzko, J.; Walker, S. The Making of a Sweet Modification: Structure and Function of O-GlcNAc Transferase. J. Biol. Chem. 2014, 289 (50), 34424-24432. [PubMed Link] [Publisher Link]
#Valentino, M. D.; #Foulston, L.; #Sadaka, A.; Kos, V. N.; Villet, R. A.; Santa Maria, J.; Lazinski, D. W.; Camilli, A.; Walker, S.; Hooper, D. C.; Gilmore, M. S. Genes Contributing to Staphylococcus aureus Fitness in Abscess- and Infection-Related Ecologies. mBio 2014, 5 (5), e01729-14. [PubMed Link] [Publisher Link]
Santa Maria, J. P.; Sadaka, A.; Moussa, S. H.; Brown, S.; Zhang, Y. J.; Rubin, E. J.; Gilmore, M. S.; Walker, S. Compound-gene interaction mapping reveals distinct roles for Staphylococcus aureus teichoic acids. Proc. Natl. Acad. Sci. U. S. A. 2014, 111 (34), 12510-12515. [PubMed Link] [Publisher Link]
#Lebar, M. D.; #May, J. M.; Meeske, A. J.; Leiman, S. A.; Lupoli, T. J.; Tsukamoto, H.; Losick, R.; Rudner, D. Z.; Walker, S.; Kahne, D. Reconstitution of Peptidoglycan Cross-Linking Leads to Improved Fluorescent Probes of Cell Wall Synthesis. J. Am. Chem. Soc. 2014, 136 (31), 10874-10877. [PubMed Link] [Publisher Link]
Sherman, D. J.; Lazarus, M. B.; Murphy, L.; Liu, C.; Walker, S.; Ruiz, N.; Kahne, D. Decoupling catalytic activity from biological function of the ATPase that powers lipopolysaccharide transport. Proc. Natl. Acad. Sci. U. S. A. 2014, 111 (13), 4982-4987. [PubMed Link] [Publisher Link]
Ortiz-Meoz, R. F.; Merbl, Y.; Kirschner, M. W.; Walker, S. Microarray Discovery of New OGT Substrates: The Medulloblastoma Oncogene OTX2 Is O-GlcNAcylated. J. Am. Chem. Soc. 2014, 136 (13), 4845-4848. [PubMed Link] [Publisher Link]
Lupoli, T. J.; Lebar, M. D.; Markovski, M.; Bernhardt, T.; Kahne, D.; Walker, S. Lipoprotein Activators Stimulate Escherichia coli Penicillin-Binding Proteins by Different Mechanisms. J. Am. Chem. Soc. 2014, 136 (1), 52-55. [PubMed Link] [Publisher Link]
Rebets, Y.; Lupoli, T.; Qiao, Y.; Schirner, K.; Villet, R.; Hooper, D.; Kahne, D.; Walker, S. Moenomycin Resistance Mutations in Staphylococcus aureus Reduce Peptidoglycan Chain Length and Cause Aberrant Cell Division. ACS Chem. Biol. 2014, 9 (2), 459-467. [PubMed Link] [Publisher Link]
2013
Makitrynskyy, R.; Ostash, B.; Tsypik, O.; Rebets, Y.; Doud, E.; Meredith, T.; Luzhetskyy, A.; Bechthold, A.; Walker, S.; Fedorenko, V. Pleiotropic regulatory genes bldA, adpA and absB are implicated in production of phosphoglycolipid antibiotic moenomycin. Open Biol. 2013, 3 (10), 130121. [PubMed Link] [Publisher Link]
Wang, H.; Gill, C. J.; Lee, S. H.; Mann, P.; Zuck, P.; Meredith, T. C.; Murgolo, N.; She, X.; Kales, S.; Liang, L.; Liu, J.; Wu, J.; Santa Maria, J.; Su, J.; Pan, J.; Hailey, J.; Mcguinness, D.; Tan, C. M.; Flattery, A.; Walker, S.; Black, T.; Roemer, T. Discovery of Wall Teichoic Acid Inhibitors as Potential Anti-MRSA β-Lactam Combination Agents. Chem. Biol. 2013, 20 (2), 272-284. [PubMed Link] [Publisher Link]
Lebar, M. D.; Lupoli, T. J.; Tsukamoto, H.; May, J. M.; Walker, S.; Kahne, D. Forming Cross-Linked Peptidoglycan from Synthetic Gram-Negative Lipid II. J. Am. Chem. Soc. 2013, 135 (12), 4632-4635. [PubMed Link] [Publisher Link]
#Lazarus, M. B.; #Jiang, J.; Kapuria, V.; Bhuiyan, T.; Janetzko, J.; Zandberg, W. F.; Vocadlo, D. J.; Herr, W.; Walker, S. HCF-1 Is Cleaved in the Active Site of O-GlcNAc Transferase. Science 2013, 342 (6163), 1235-1239. [PubMed Link] [Publisher Link]
Garg, N.; Oman, T. J.; Wang, T.-S. A.; De Gonzalo, C. V. G.; Walker, S.; van der Donk, W. A. Mode of action and structure-activity relationship studies of geobacillin I. J. Antibiot. (Tokyo) 2013, 67 (1), 133-136. [PubMed Link] [Publisher Link]
Ostash, B.; Campbell, J.; Luzhetskyy, A.; Walker, S. MoeH5: a natural glycorandomizer from the moenomycin biosynthetic pathway. Mol. Microbiol. 2013, 90 (6), 1324-1338. [PubMed Link] [Publisher Link]
Pasquina, L. W.; Santa Maria, J. P.; Walker, S. Teichoic acid biosynthesis as an antibiotic target. Curr. Opin. Microbiol. 2013, 16 (5), 531-537. [PubMed Link] [Publisher Link]
Gampe, C. M.; Tsukamoto, H.; Doud, E. H.; Walker, S.; Kahne, D. Tuning the Moenomycin Pharmacophore To Enable Discovery of Bacterial Cell Wall Synthesis Inhibitors. J. Am. Chem. Soc. 2013, 135 (10), 3776-3779. [PubMed Link] [Publisher Link]
Brown, S.; Santa Maria, J. P.; Walker, S. Wall Teichoic Acids of Gram-Positive Bacteria. Annu. Rev. Microbiol. 2013, 67, 313-336. [PubMed Link] [Publisher Link]
2012
#Jiang, J.; #Lazarus, M. B.; Pasquina, L.; Sliz, P.; Walker, S. A neutral diphosphate mimic crosslinks the active site of human O-GlcNAc transferase. Nat. Chem. Biol. 2012, 8 (1), 72-77. [PubMed Link] [Publisher Link]
Palmer, K. L.; Godfrey, P.; Griggs, A.; Kos, V. N.; Zucker, J.; Desjardins, C.; Cerqueira, G.; Gevers, D.; Walker, S.; Wortman, J.; Feldgarden, M.; Haas, B.; Birren, B.; Gilmore, M. S. Comparative Genomics of Enterococci: Variation in Enterococcus faecalis, Clade Structure in E. faecium, and Defining Characteristics of E. gallinarum and E. casseliflavus. mBio 2012, 3 (1), e00318-11. [PubMed Link] [Publisher Link]
#Knerr, P. J.; #Oman, T. J.; De Gonzalo, C. V. G.; Lupoli, T. J.; Walker, S.; van der Donk, W. A. Non-proteinogenic Amino Acids in Lacticin 481 Analogues Result in More Potent Inhibition of Peptidoglycan Transglycosylation. ACS Chem. Biol. 2012, 7 (11), 1791-1795. [PubMed Link] [Publisher Link]
#Lazarus, M. B.; #Jiang, J.; Gloster, T. M.; Zandberg, W. F.; Whitworth, G. E.; Vocadlo, D. J.; Walker, S. Structural snapshots of the reaction coordinate for O-GlcNAc transferase. Nat. Chem. Biol. 2012, 8 (12), 966-968. [PubMed Link] [Publisher Link]
*Ostash, B.; Doud, E.; Walker, S. ABC transporter genes from Streptomyces ghanaensis moenomycin biosynthetic gene cluster: roles in antibiotic production and export. Arch. Microbiol. 2012, 194 (11), 915-922. [PubMed Link] [Publisher Link]
#Campbell, J.; #Singh, A. K.; Swoboda, J. G.; Gilmore, M. S.; Wilkinson, B. J.; Walker, S. An Antibiotic That Inhibits a Late Step in Wall Teichoic Acid Biosynthesis Induces the Cell Wall Stress Stimulon in Staphylococcus aureus. Antimicrob. Agents Chemother. 2012, 56 (4), 1810-1820. [PubMed Link] [Publisher Link]
Brown, S.; Xia, G.; Luhachack, L. G.; Campbell, J.; Meredith, T. C.; Chen, C.; Winstel, V.; Gekeler, C.; Irazoqui, J. E.; Peschel, A.; Walker, S. Methicillin resistance in Staphylococcus aureus requires glycosylated wall teichoic acids. Proc. Natl. Acad. Sci. U. S. A. 2012, 109 (46), 18909-18914. [PubMed Link] [Publisher Link]
Suzuki, T.; Campbell, J.; Kim, Y.; Swoboda, J. G.; Mylonakis, E.; Walker, S.; Gilmore, M. S. Wall teichoic acid protects Staphylococcus aureus from inhibition by Congo red and other dyes. J. Antimicrob. Chemother. 2012, 67 (9), 2143-2151. [PubMed Link] [Publisher Link]
2011
Suzuki, T.; Swoboda, J. G.; Campbell, J.; Walker, S.; Gilmore, M. S. In vitro Antimicrobial Activity of Wall Teichoic Acid Biosynthesis Inhibitors against Staphylococcus aureus Isolates. Antimicrob. Agents Chemother. 2011, 55 (2), 767-774. [PubMed Link] [Publisher Link]
Campbell, J.; Singh, A. K.; Santa Maria, J. P.; Kim, Y.; Brown, S.; Swoboda, J. G.; Mylonakis, E.; Wilkinson, B. J.; Walker, S. Synthetic Lethal Compound Combinations Reveal a Fundamental Connection between Wall Teichoic Acid and Peptidoglycan Biosynthesis in Staphylococcus aureus. ACS Chem. Biol. 2011, 6 (1), 106-116. [PubMed Link] [Publisher Link]
Schirner, K.; Stone, L. K.; Walker, S. ABC Transporters Required for Export of Wall Teichoic Acids Do Not Discriminate between Different Main Chain Polymers. ACS Chem. Biol. 2011, 6 (5), 407-412. [PubMed Link] [Publisher Link]
#Ostash, B.; #Rebets, Y.; Myronovskyy, M.; Tsypik, O.; Ostash, I.; Kulachkovskyy, O.; Datsyuk, Y.; Nakamura, T.; Walker, S.; Fedorenko, V. Identification and characterization of the Streptomyces globisporus 1912 regulatory gene lndYR that affects sporulation and antibiotic production. Microbiology 2011, 157 (4), 1240-1249. [PubMed Link] [Publisher Link]
Suzuki, T.; Campbell, J.; Swoboda, J. G.; Walker, S.; Gilmore, M. S. Role of Wall Teichoic Acids in Staphylococcus aureus Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2011, 52 (6), 3187-3192. [PubMed Link] [Publisher Link]
Rabyk, M.; Ostash, B.; Rebets, Y.; Walker, S.; Fedorenko, V. Streptomyces ghanaensis pleiotropic regulatory gene wblA(gh) influences morphogenesis and moenomycin production. Biotechnol. Lett. 2011, 33 (12), 2481-2486. [PubMed Link] [Publisher Link]
#Lazarus, M. B.; #Nam, Y.; Jiang, J.; Sliz, P.; Walker, S. Structure of human O-GlcNAc transferase and its complex with a peptide substrate. Nature 2011, 469 (7331), 564-567. [PubMed Link] [Publisher Link]
Doud, E. H.; Perlstein, D. L.; Wolpert, M.; Cane, D. E.; Walker, S. Two Distinct Mechanisms for TIM Barrel Prenyltransferases in Bacteria. J. Am. Chem. Soc. 2011, 133 (5), 1270-1273. [PubMed Link] [Publisher Link]
Oman, T. J.; Lupoli, T. J.; Wang, T.-S. A.; Kahne, D.; Walker, S.; van der Donk, W. A. Haloduracin α Binds the Peptidoglycan Precursor Lipid II with 2:1 Stoichiometry. J. Am. Chem. Soc. 2011, 133 (44), 17544-17547. [PubMed Link] [Publisher Link]
Gampe, C. M.; Tsukamoto, H.; Wang, T.-S. A.; Walker, S.; Kahne, D. Modular synthesis of diphospholipid oligosaccharide fragments of the bacterial cell wall and their use to study the mechanism of moenomycin and other antibiotics. Tetrahedron 2011, 67 (51), 9771-9778. [PubMed Link] [Publisher Link]
Wang, T.-S. A.; Lupoli, T. J.; Sumida, Y.; Tsukamoto, H.; Wu, Y.; Rebets, Y.; Kahne, D. E.; Walker, S. Primer Preactivation of Peptidoglycan Polymerases. J. Am. Chem. Soc. 2011, 133 (22), 8528-8530. [PubMed Link] [Publisher Link]
Lupoli, T. J.; Tsukamoto, H.; Doud, E. H.; Wang, T.-S. A.; Walker, S.; Kahne, D. Transpeptidase-Mediated Incorporation of D-Amino Acids into Bacterial Peptidoglycan. J. Am. Chem. Soc. 2011, 133 (28), 10748-10751. [PubMed Link] [Publisher Link]
2010
#Caldwell, S. A.; #Jackson, S. R.; #Shahriari, K. S.; Lynch, T. P.; Sethi, G.; Walker, S.; Vosseller, K.; Reginato, M. J. Nutrient sensor O-GlcNAc transferase regulates breast cancer tumorigenesis through targeting of the oncogenic transcription factor FoxM1. Oncogene 2010, 29 (19), 2831-2842. [PubMed Link] [Publisher Link]
Swoboda, J. G.; Campbell, J.; Meredith, T. C.; Walker, S. Wall Teichoic Acid Function, Biosynthesis, and Inhibition. Chembiochem 2010, 11 (1), 35-45. [PubMed Link] [Publisher Link]
Brown, S.; Meredith, T.; Swoboda, J.; Walker, S. Staphylococcus aureus and Bacillus subtilis W23 Make Polyribitol Wall Teichoic Acids Using Different Enzymatic Pathways. Chem. Biol. 2010, 17 (10), 1101-1110. [PubMed Link] [Publisher Link]
Lee, K.; Campbell, J.; Swoboda, J. G.; Cuny, G. D.; Walker, S. Development of improved inhibitors of wall teichoic acid biosynthesis with potent activity against Staphylococcus aureus. Bioorg. Med. Chem. Lett. 2010, 20 (5), 1767-1770. [PubMed Link] [Publisher Link]
Makitrynskyy, R.; Rebets, Y.; Ostash, B.; Zaburannyi, N.; Rabyk, M.; Walker, S.; Fedorenko, V. Genetic factors that influence moenomycin production in streptomycetes. J. Ind. Microbiol. Biotechnol. 2010, 37 (6), 559-566. [PubMed Link] [Publisher Link]
Ostash, B.; Walker, S. Moenomycin family antibiotics: chemical synthesis, biosynthesis, and biological activity. Nat. Prod. Rep. 2010, 27 (11), 1594-1617. [PubMed Link] [Publisher Link]
Silhavy, T. J.; Kahne, D.; Walker, S. The Bacterial Cell Envelope. Cold Spring Harb. Perspect. Biol. 2010, 2 (5), a000414. [PubMed Link] [Publisher Link]
Fuse, S.; Tsukamoto, H.; Yuan, Y.; Wang, T.-S. A.; Zhang, Y.; Bolla, M.; Walker, S.; Sliz, P.; Kahne, D. Functional and Structural Analysis of a Key Region of the Cell Wall Inhibitor Moenomycin. ACS Chem. Biol. 2010, 5 (7), 701-711. [PubMed Link] [Publisher Link]
#Paradis-Bleau, C.; #Markovski, M.; #Uehara, T.; Lupoli, T. J.; Walker, S.; Kahne, D. E.; Bernhardt, T. G. Lipoprotein Cofactors Located in the Outer Membrane Activate Bacterial Cell Wall Polymerases. Cell 2010, 143 (7), 1110-1120. [PubMed Link] [Publisher Link]
Perlstein, D. L.; Wang, T.-S. A.; Doud, E. H.; Kahne, D.; Walker, S. The Role of the Substrate Lipid in Processive Glycan Polymerization by the Peptidoglycan Glycosyltransferases. J. Am. Chem. Soc. 2010, 132 (1), 48-49. [PubMed Link] [Publisher Link]
#Shi, Y.; #Tomic, J.; Wen, F.; Shaha, S.; Bahlo, A.; Harrison, R.; Dennis, J. W.; Williams, R.; Gross, B. J.; Walker, S.; Zuccolo, J.; Deans, J. P.; Hart, G. W.; Spaner, D. E. Aberrant O-GlcNAcylation characterizes chronic lymphocytic leukemia. Leukemia 2010, 24 (9), 1588-1598. [PubMed Link] [Publisher Link]
Ostash, B.; Ostash, I.; Zhu, L.; Kharel, M. K.; Luzhetskyy, A.; Bechthold, A.; Walker, S.; Rohr, J.; Fedorenko, V. Properties of lanK-based regulatory circuit involved in landomycin biosynthesis in Streptomyces cyanogenus S136. Genetika 2010, 46 (5), 604-609. [PubMed Link] [Publisher Link]
2009
Swoboda, J. G.; Meredith, T. C.; Campbell, J.; Brown, S.; Suzuki, T.; Bollenbach, T.; Malhowski, A. J.; Kishony, R.; Gilmore, M. S.; Walker, S. Discovery of a Small Molecule that Blocks Wall Teichoic Acid Biosynthesis in Staphylococcus aureus. ACS Chem. Biol. 2009, 4 (10), 875-883. [PubMed Link] [Publisher Link]
Fang, X.; Nam, J.; Shin, D.; Rew, Y.; Boger, D. L.; Walker, S. Functional and biochemical analysis of a key series of ramoplanin analogues. Bioorg. Med. Chem. Lett. 2009, 19 (21), 6189-6191. [PubMed Link] [Publisher Link]
Ostash, B.; Makitrinskyy, R.; Walker, S.; Fedorenko, V. Identification and characterization of Streptomyces ghanaensis ATCC14672 integration sites for three actinophage-based plasmids. Plasmid 2009, 61 (3), 171-175. [PubMed Link] [Publisher Link]
Ostash, B.; Doud, E. H.; Lin, C.; Ostash, I.; Perlstein, D. L.; Fuse, S.; Wolpert, M.; Kahne, D.; Walker, S. Complete Characterization of the Seventeen Step Moenomycin Biosynthetic Pathway. Biochemistry 2009, 48 (37), 8830-8841. [PubMed Link] [Publisher Link]
Lupoli, T. J.; Taniguchi, T.; Wang, T.-S.; Perlstein, D. L.; Walker, S.; Kahne, D. E. Studying a Cell Division Amidase Using Defined Peptidoglycan Substrates. J. Am. Chem. Soc. 2009, 131 (51), 18230-18231. [PubMed Link] [Publisher Link]
2008
Ostash, I.; Rebets, Y.; Ostash, B.; Kobylyanskyy, A.; Myronovskyy, M.; Nakamura, T.; Walker, S.; Fedorenko, V. An ABC transporter encoding gene lndW confers resistance to landomycin E. Arch. Microbiol. 2008, 190 (1), 105-109. [PubMed Link] [Publisher Link]
Ostash, I.; Ostash, B.; Luzhetskyy, A.; Bechthold, A.; Walker, S.; Fedorenko, V. Coordination of export and glycosylation of landomycins in Streptomyces cyanogenus S136. FEMS Microbiol. Lett. 2008, 285 (2), 195-202. [PubMed Link] [Publisher Link]
Meredith, T. C.; Swoboda, J. G.; Walker, S. Late-Stage Polyribitol Phosphate Wall Teichoic Acid Biosynthesis in Staphylococcus aureus. J. Bacteriol. 2008, 190 (8), 3046-3056. [PubMed Link] [Publisher Link]
Brown, S.; Zhang, Y.-H.; Walker, S. A Revised Pathway Proposed for Staphylococcus aureus Wall Teichoic Acid Biosynthesis Based on In Vitro Reconstitution of the Intracellular Steps. Chem. Biol. 2008, 15 (1), 12-21. [PubMed Link] [Publisher Link]
Gross, B. J.; Swoboda, J. G.; Walker, S. A Strategy to Discover Inhibitors of O-Linked Glycosylation. J. Am. Chem. Soc. 2008, 130 (2), 440-441. [PubMed Link] [Publisher Link]
Wang, T.-S. A.; Manning, S. A.; Walker, S.; Kahne, D. Isolated Peptidoglycan Glycosyltransferases from Different Organisms Produce Different Glycan Chain Lengths. J. Am. Chem. Soc. 2008, 130 (43), 14068-14069. [PubMed Link] [Publisher Link]
Yuan, Y.; Fuse, S.; Ostash, B.; Sliz, P.; Kahne, D.; Walker, S. Structural Analysis of the Contacts Anchoring Moenomycin to Peptidoglycan Glycosyltransferases and Implications for Antibiotic Design. ACS Chem. Biol. 2008, 3 (7), 429-436. [PubMed Link] [Publisher Link]
2007
Dehennaut, V.; Lefebvre, T.; Sellier, C.; Leroy, Y.; Gross, B.; Walker, S.; Cacan, R.; Michalski, J.-C.; Vilain, J.-P.; Bodart, J.-F. O-Linked N-Acetylglucosaminyltransferase Inhibition Prevents G(2)/M Transition in Xenopus laevis Oocytes. J. Biol. Chem. 2007, 282 (17), 12527-12536. [PubMed Link] [Publisher Link]
Ostash, B.; Saghatelian, A.; Walker, S. A Streamlined Metabolic Pathway for the Biosynthesis of Moenomycin A. Chem. Biol. 2007, 14 (3), 257-267. [PubMed Link] [Publisher Link]
Barrett, D.; Wang, T.-S. A.; Yuan, Y.; Zhang, Y.; Kahne, D.; Walker, S. Analysis of Glycan Polymers Produced by Peptidoglycan Glycosyltransferases. J. Biol. Chem. 2007, 282 (44), 31964-31971. [PubMed Link] [Publisher Link]
Leimkuhler, C.; Fridman, M.; Lupoli, T.; Walker, S.; Walsh, C. T.; Kahne, D. Characterization of Rhodosaminyl Transfer by the AknS/AknT Glycosylation Complex and Its Use in Reconstituting the Biosynthetic Pathway of Aclacinomycin A. J. Am. Chem. Soc. 2007, 129 (34), 10546-10550. [PubMed Link] [Publisher Link]
Yuan, Y.; Barrett, D.; Zhang, Y.; Kahne, D.; Sliz, P.; Walker, S. Crystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis. Proc. Natl. Acad. Sci. U. S. A. 2007, 104 (13), 5348-5353. [PubMed Link] [Publisher Link]
Perlstein, D. L.; Zhang, Y.; Wang, T.-S.; Kahne, D. E.; Walker, S. The Direction of Glycan Chain Elongation by Peptidoglycan Glycosyltransferases. J. Am. Chem. Soc. 2007, 129 (42), 12674-12675. [PubMed Link] [Publisher Link]
Zhang, Y.; Fechter, E. J.; Wang, T.-S. A.; Barrett, D.; Walker, S.; Kahne, D. E. Synthesis of Heptaprenyl-Lipid IV to Analyze Peptidoglycan Glycosyltransferases. J. Am. Chem. Soc. 2007, 129 (11), 3080-3081. [PubMed Link] [Publisher Link]
Ostash, I.; Ostash, B.; Walker, S.; Fedorenko, V. Proton-dependent transporter gene lndJ confers resistance to landomycin E in Streptomyces globisporus 1912. Genetika 2007, 43 (8), 1032-1037. [PubMed Link] [Publisher Link]
2006
Zhang, Y.-H.; Ginsberg, C.; Yuan, Y.; Walker, S. Acceptor Substrate Selectivity and Kinetic Mechanism of Bacillus subtilis TagA. Biochemistry 2006, 45 (36), 10895-10904. [PubMed Link] [Publisher Link]
Love, K. R.; Swoboda, J. G.; Noren, C. J.; Walker, S. Enabling Glycosyltransferase Evolution: A Facile Substrate-Attachment Strategy for Phage-Display Enzyme Evolution. Chembiochem 2006, 7 (5), 753-756. [PubMed Link] [Publisher Link]
Tiyanont, K.; Doan, T.; Lazarus, M. B.; Fang, X.; Rudner, D. Z.; Walker, S. Imaging peptidoglycan biosynthesis in Bacillus subtilis with fluorescent antibiotics. Proc. Natl. Acad. Sci. U. S. A. 2006, 103 (29), 11033-11038. [PubMed Link] [Publisher Link]
Ginsberg, C.; Zhang, Y.-H.; Yuan, Y.; Walker, S. In Vitro Reconstitution of Two Essential Steps in Wall Teichoic Acid Biosynthesis. ACS Chem. Biol. 2006, 1 (1), 25-28. [PubMed Link] [Publisher Link]
Fang, X.; Tiyanont, K.; Zhang, Y.; Wanner, J.; Boger, D.; Walker, S. The mechanism of action of ramoplanin and enduracin. Mol. Biosyst. 2006, 2 (1), 69-76. [PubMed Link] [Publisher Link]
2005
Ostash, B.; Walker, S. Bacterial transglycosylase inhibitors. Curr. Opin. Chem. Biol. 2005, 9 (5), 459-466. [PubMed Link] [Publisher Link]
Walker, S.; Chen, L.; Hu, Y.; Rew, Y.; Shin, D.; Boger, D. L. Chemistry and Biology of Ramoplanin: A Lipoglycodepsipeptide with Potent Antibiotic Activity. Chem. Rev. 2005, 105 (2), 449-476. [PubMed Link] [Publisher Link]
Gross, B. J.; Kraybill, B. C.; Walker, S. Discovery of O-GlcNAc Transferase Inhibitors. J. Am. Chem. Soc. 2005, 127 (42), 14588-14589. [PubMed Link] [Publisher Link]
Barrett, D.; Leimkuhler, C.; Chen, L.; Walker, D.; Kahne, D.; Walker, S. Kinetic Characterization of the Glycosyltransferase Module of Staphylococcus aureus PBP2. J. Bacteriol. 2005, 187 (6), 2215-2217. [PubMed Link] [Publisher Link]
Leimkuhler, C.; Chen, L.; Barrett, D.; Panzone, G.; Sun, B.; Falcone, B.; Oberthür, M.; Donadio, S.; Walker, S.; Kahne, D. Differential Inhibition of Staphylococcus aureus PBP2 by Glycopeptide Antibiotics. J. Am. Chem. Soc. 2005, 127 (10), 3250-3251. [PubMed Link] [Publisher Link]
Yuan, Y.; Chung, H. S.; Leimkuhler, C.; Walsh, C. T.; Kahne, D.; Walker, S. In Vitro Reconstitution of EryCIII Activity for the Preparation of Unnatural Macrolides. J. Am. Chem. Soc. 2005, 127 (41), 14128-14129. [PubMed Link] [Publisher Link]
2004
Chen, L.; Yuan, Y.; Helm, J. S.; Hu, Y.; Rew, Y.; Shin, D.; Boger, D. L.; Walker, S. Dissecting Ramoplanin: Mechanistic Analysis of Synthetic Ramoplanin Analogues as a Guide to the Design of Improved Antibiotics. J. Am. Chem. Soc. 2004, 126 (24), 7462-7463. [PubMed Link] [Publisher Link]
Barrett, D. S.; Chen, L.; Litterman, N. K.; Walker, S. Expression and Characterization of the Isolated Glycosyltransferase Module of Escherichia coli PBP1b. Biochemistry 2004, 43 (38), 12375-12381. [PubMed Link] [Publisher Link]
Hu, Y.; Helm, J. S.; Chen, L.; Ginsberg, C.; Gross, B.; Kraybill, B.; Tiyanont, K.; Fang, X.; Wu, T.; Walker, S. Identification of Selective Inhibitors for the Glycosyltransferase MurG via High-Throughput Screening. Cell Chem. Biol. 2004, 11 (5), 703-711. [PubMed Link] [Publisher Link]
Garneau, S.; Qiao, L.; Chen, L.; Walker, S.; Vederas, J. C. Synthesis of mono- and disaccharide analogs of moenomycin and lipid II for inhibition of transglycosylase activity of penicillin-binding protein 1b. Bioorg. Med. Chem. 2004, 12 (24), 6473-6494. [PubMed Link] [Publisher Link]
Lee, H. Y.; Chung, H. S.; Hang, C.; Khosla, C.; Walsh, C. T.; Kahne, D.; Walker, S. Reconstitution and Characterization of a New Desosaminyl Transferase, EryCIII, from the Erythromycin Biosynthetic Pathway. J. Am. Chem. Soc. 2004, 126 (32), 9924-9925. [PubMed Link] [Publisher Link]
2003
Hu, Y.; Chen, L.; Ha, S.; Gross, B.; Falcone, B.; Walker, D.; Mokhtarzadeh, M.; Walker, S. Crystal structure of the MurG:UDP-GlcNAc complex reveals common structural principles of a superfamily of glycosyltransferases. Proc. Natl. Acad. Sci. U. S. A. 2003, 100 (3), 845-859. [PubMed Link] [Publisher Link]
Breukink, E.; van Heusden, H. E.; Vollmerhaus, P. J.; Swiezewska, E.; Brunner, L.; Walker, S.; Heck, A. J. R.; de Kruijff, B. Lipid II Is an Intrinsic Component of the Pore Induced by Nisin in Bacterial Membranes. J. Biol. Chem. 2003, 278 (22), 19898-19903. [PubMed Link] [Publisher Link]
Hu, Y.; Helm, J. S.; Chen, L.; Ye, X.-Y.; Walker, S. Ramoplanin Inhibits Bacterial Transglycosylases by Binding as a Dimer to Lipid II. J. Am. Chem. Soc. 2003, 125 (29), 8736-8737. [PubMed Link] [Publisher Link]
Chen, L.; Walker, D.; Sun, B.; Hu, Y.; Walker, S.; Kahne, D. Vancomycin analogues active against vanA-resistant strains inhibit bacterial transglycosylase without binding substrate. Proc. Natl. Acad. Sci. U. S. A. 2003, 100 (10), 5658-5663. [PubMed Link] [Publisher Link]
Helm, J. S.; Hu, Y.; Chen, L.; Gross, B.; Walker, S. Identification of Active-Site Inhibitors of MurG Using a Generalizable, High-Throughput Glycosyltransferase Screen. J. Am. Chem. Soc. 2003, 125 (37), 11168-11169. [PubMed Link] [Publisher Link]
2002
Chen, L.; Men, H.; Ha, S.; Ye, X.-Y.; Brunner, L.; Hu, Y.; Walker, S. Intrinsic Lipid Preferences and Kinetic Mechanism of Escherichia coli MurG. Biochemistry 2002, 41 (21), 6824-6833. [PubMed Link] [Publisher Link]
Hu, Y.; Walker, S. Remarkable Structural Similarities between Diverse Glycosyltransferases. Cell Chem. Biol. 2002, 9 (12), 1287-1296. [PubMed Link] [Publisher Link]
Helm, J. S.; Chen, L.; Walker, S. Rethinking Ramoplanin: The Role of Substrate Binding in Inhibition of Peptidoglycan Biosynthesis. J. Am. Chem. Soc. 2002, 124 (47), 13970-13971. [PubMed Link] [Publisher Link]
Lazar, K.; Walker, S. Substrate analogues to study cell-wall biosynthesis and its inhibition. Curr. Opin. Chem. Biol. 2002, 6 (6), 786-793. [PubMed Link] [Publisher Link]
2001
Ye, X.-Y.; Lo, M.-C.; Brunner, L.; Walker, D.; Kahne, D.; Walker, S. Better Substrates for Bacterial Transglycosylases. J. Am. Chem. Soc. 2001, 123 (13), 3155-3156. [PubMed Link] [Publisher Link]
Ha, S.; Gross, B.; Walker, S. E. coli MurG: A Paradigm for a Superfamily of Glycosyltransferases. Curr. Drug Targets Infect. Disord. 2001, 1 (2), 201-213. [PubMed Link] [Publisher Link]
Lo, M.-C.; Helm, J. S.; Sarngadharan, G.; Pelczer, I.; Walker, S. A New Structure for the Substrate-Binding Antibiotic Ramoplanin. J. Am. Chem. Soc. 2001, 123 (35), 8640-8641. [PubMed Link] [Publisher Link]
2000
Ha, S.; Walker, D.; Shi, Y.; Walker, S. The 1.9 Å crystal structure of Escherichia coli MurG, a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis. Protein Sci. 2000, 9 (6), 1045-1052. [PubMed Link] [Publisher Link]
Lo, M.-C.; Men, H.; Branstrom, A.; Helm, J.; Yao, N.; Goldman, R.; Walker, S. A New Mechanism of Action Proposed for Ramoplanin. J. Am. Chem. Soc. 2000, 122 (14), 3540-3541. [Publisher Link]
pre-2000
Ha, S.; Chang, E.; Lo, M.-C.; Men, H.; Park, P.; Ge, M.; Walker, S. The Kinetic Characterization of Escherichia coli MurG Using Synthetic Substrate Analogues. J. Am. Chem. Soc. 1999, 121 (37), 8415-8426. [Publisher Link]
Lo, M.-C.; Ha, S.; Pelczer, I.; Pal, S.; Walker, S. The solution structure of the DNA-binding domain of Skn-1. Proc. Natl. Acad. Sci. U. S. A. 1998, 95 (15), 8455-8460. [PubMed Link] [Publisher Link]
Men, H.; Park, P.; Ge, M.; Walker, S. Substrate Synthesis and Activity Assay for MurG. J. Am. Chem. Soc. 1998, 120 (10), 2484-2485. [Publisher Link]
Pal, S.; Lo, M.-C.; Schmidt, D.; Pelczer, I.; Thurber, S.; Walker, S. Skn-1: Evidence for a bipartite recognition helix in DNA binding. Proc. Natl. Acad. Sci. U. S. A. 1997, 94 (11), 5556-5561. [PubMed Link] [Publisher Link]