“#” denotes co-first authorship
2024
Bartlett, T. M.; Sisley, T. A.; Mychack, A.; Walker, S.; Baker, R. W.; *Rudner, D. Z.; *Bernhardt, T. G. FacZ is a GpsB-interacting protein that prevents aberrant division-site placement in Staphylococcus aureus. Nat. Microbiol. 2024, 9, 801-813. [PubMed Link] [Publisher Link]
2023
Schultz, B. J.; Snow, E. D.; Walker, S. Mechanism of ᴅ-alanine transfer to teichoic acids shows how bacteria acylate cell envelope polymers. Nat. Microbiol. 2023. [PubMed Link] [Publisher Link]
Shlosman, I.; Fivenson, E. M.; Gilman, M. S. A.; Sisley, T. A.; Walker, S.; Bernhardt, T. G.; *Kruse, A. C.; *Loparo, J. J. Allosteric activation of cell wall synthesis during bacterial growth. Nat. Commun. 2023. [PubMed Link] [Publisher Link]
#Park, Y.; #Taguchi, A.; Baidin, V.; Kahne, D.; Walker, S. A Time-Resolved FRET Assay Identifies a Small Molecule that Inhibits the Essential Bacterial Cell Wall Polymerase FtsW. Angew. Chem. Int. Ed. 2023, e202301522. [PubMed Link] [Publisher Link]
Stone, M. C.; Mychack, A.; Coe, K. A.; Walker, S. Combining Signal Peptidase and Lipoprotein Processing Inhibitors Overcomes Ayr Resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 2023, e00115-23. [PubMed Link] [Publisher Link]
Hummels, K. R.; Berry, S. P.; Li, Z.; Taguchi, A.; Min, J. K.; Walker, S.; Marks, D. S.; Bernhardt, T. G. Coordination of bacterial cell wall and outer membrane biosynthesis. Nature 2023, 615 (7951), 300-304. [PubMed Link] [Publisher Link]
2022
Page, J. E.; Skiba, M. A.; Do, T.; Walker, S. Metal cofactor stabilization by a partner protein is a widespread strategy employed for amidase activation. Proc. Natl. Acad. Sci. U. S. A. 2022, 119 (26), e2201141119. [PubMed Link] [Publisher Link]
Gondane, A.; Poulose, N.; Walker, S.; Mills, I. G.; Itkonen, H. M. O-GlcNAc transferase maintains metabolic homeostasis in response to CDK9 inhibition. Glycobiology 2022, 00, 1-9. [PubMed Link] [Publisher Link]
Muscato, J. D.; Morris, H. G.; Mychack, A.; Rajagopal, M.; Baidin, V.; Hesser, A.; Lee, W.; İnecik, K.; Wilson, L. J.; Kraml, C. M.; Meredith, T. C.; Walker, S. Rapid inhibitor discovery by exploiting synthetic lethality. J. Am. Chem. Soc. 2022, 144 (8), 3696-3705. [PubMed Link] [Publisher Link]
2021
Taguchi, A.; Page, J. E.; Tsui, H.-C. T.; Winkler, M. E.; Walker, S. Biochemical reconstitution defines new functions for membrane-bound glycosidases in assembly of the bacterial cell wall. Proc. Natl. Acad. Sci. U. S. A. 2021, 118 (36), e2103740118. [PubMed Link] [Publisher Link]
*Joiner, C. M.; Hammel, F. A.; Janetzko, J.; *Walker, S. Protein Substrates Engage the Lumen of O-GlcNAc Transferase’s Tetratricopeptide Repeat Domain in Different Ways. Biochemistry 2021, 60 (11), 847-853. [PubMed Link] [Publisher Link]
Page, J. E.; Walker, S. Natural products that target the cell envelope. Curr. Opin. Microbiol. 2021, 61, 16-24. [PubMed Link] [Publisher Link]
Levine, Z. G.; Potter, S. C.; Joiner, C. M.; Fei, G. Q.; Nabet, B.; Sonnett, M.; Zachara, N. E.; Gray, N. S.; Paulo, J. A.; Walker, S. Mammalian cell proliferation requires noncatalytic functions of O-GlcNAc transferase. Proc. Natl. Acad. Sci. U. S. A. 2021, 118 (4), e2016778118. [PubMed Link] [Publisher Link]
#Schaefer, K.; #Owens, T. W.; Page, J. E.; Santiago, M.; Kahne, D.; Walker, S. Structure and reconstitution of a hydrolase complex that may release peptidoglycan from the membrane after polymerization. Nat. Microbiol. 2021, 6, 34-43. [PubMed Link] [Publisher Link]
2020
Hesser, A. R.; Schaefer, K.; Lee, W.; Walker, S. Lipoteichoic acid polymer length is determined by competition between free starter units. Proc. Natl. Acad. Sci. U. S. A. 2020, 117 (47), 29669-29676. [PubMed Link] [Publisher Link]
Itkonen, H. M.; Poulose, N.; Steele, R. E.; Martin, S. E. S.; Levine, Z. G.; Duveau, D. Y.; Carelli, R.; Singh, R.; Urbanucci, A.; Loda, M.; Thomas, C. J.; Mills, I. G.; Walker, S. Inhibition of O-GlcNAc Transferase Renders Prostate Cancer Cells Dependent on CDK9. Mol. Cancer Res. 2020, 18 (10), 1512-1521. [PubMed Link] [Publisher Link]
#Hesser, A. R.; #Matano, L. M.; #Vickery, C. R.; Wood, B. M.; Santiago, A. G.; Morris, H. G.; Do, T.; Losick, R.; Walker, S. The Length of Lipoteichoic Acid Polymers Controls Staphylococcus aureus Cell Size and Envelope Integrity. J. Bacteriol. 2020, 202 (16), e00149-20. [PubMed Link] [Publisher Link]
#Tan, Z.-W.; #Fei, G.; Paulo, J. A.; Bellaousov, S.; Martin, S. E. S.; Duveau, D. Y.; Thomas, C. J.; Gygi, S. P.; *Boutz, P. L.; *Walker, S. O-GlcNAc regulates gene expression by controlling detained intron splicing. Nucleic Acids Res. 2020, 48 (10), 5656-5669. [PubMed Link] [Publisher Link]
Laut, C. L.; Perry, W. J.; Metzger, A. L.; Weiss, A.; Stauff, D. L.; Walker, S.; Caprioli, R. M.; Skaar, E. P. Bacillus anthracis Responds to Targocil-Induced Envelope Damage through EdsRS Activation of Cardiolipin Synthesis. mBio 2020, 11 (2), e03375-19. [PubMed Link] [Publisher Link]
Sjodt, M.; Rohs, P. D. A.; Gilman, M. S. A.; Erlandson, S. C.; Zheng, S.; Green, A. G.; Brock, K. P.; Taguchi, A.; Kahne, D.; Walker, S.; Marks, D. S.; Rudner, D. Z.; Bernhardt, T. G.; Kruse, A. C. Structural coordination of polymerization and crosslinking by a SEDS-bPBP peptidoglycan synthase complex. Nat. Microbiol. 2020, 5 (6), 813-820. [PubMed Link] [Publisher Link]
#Rubino, F. A.; #Mollo, A.; Kumar, S.; Butler, E. K.; *Ruiz, N.; *Walker, S.; *Kahne, D. E. Direction of Transport Intermediates in the Peptidoglycan Flippase MurJ Identifies Residues Essential for Conformational Cycling. J. Am. Chem. Soc. 2020, 142 (12), 5482-5486. [PubMed Link] [Publisher Link]
Do, T.; Page, J. E.; Walker, S. Uncovering the activities, biological roles, and regulation of bacterial cell wall hydrolases and tailoring enzymes. J. Biol. Chem. 2020, 295 (10), 3347-3361. [PubMed Link] [Publisher Link]
Palace, S. G.; Wang, Y.; Rubin, D. H. F.; Welsh, M. A.; Mortimer, T. D.; Cole, K.; Eyre, D. W.; Walker, S.; Grad, Y. H. RNA polymerase mutations cause cephalosporin resistance in clinical Neisseria gonorrhoeae isolates. eLife 2020, 9, e51407. [PubMed Link] [Publisher Link]
Do, T.; Schaefer, K.; Santiago, A. G.; Coe, K. A.; Fernandes, P. B.; Kahne, D.; Pinho, M. G.; Walker, S. Staphylococcus aureus cell growth and division are regulated by an amidase that trims peptides from uncrosslinked peptidoglycan. Nat. Microbiol. 2020, 5 (2), 291-303. [PubMed Link] [Publisher Link]
2019
Schuster, C. F.; Wiedemann, D. M.; Kirsebom, F. C. M.; Santiago, M.; Walker, S.; Gründling, A. High-throughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistant Staphylococcus aureus and underlies a tailored response to different osmotic stressors. Mol. Microbiol. 2019, xx (xx), x-x. [PubMed Link] [Publisher Link]
#Coe, K. A.; #Lee, W.; Stone, M. C.; Komazin-Meredith, G.; *Meredith, T. C.; *Grad, Y. H.; *Walker, S. Multi-strain Tn-Seq reveals common daptomycin resistance determinants in Staphylococcus aureus. PLoS Pathog. 2019, 15 (11), e1007862. [PubMed Link] [Publisher Link]
#Joiner, C. M.; #Levine, Z. G.; Aonbangkhen, C.; Woo, C. M.; Walker, S. Aspartate Residues Far from the Active Site Drive O-GlcNAc Transferase Substrate Selection. J. Am. Chem. Soc. 2019, 141 (33), 12974-12978. [PubMed Link] [Publisher Link]
Taguchi, A.; Kahne, D.; Walker, S. Chemical tools to characterize peptidoglycan synthases. Curr. Opin. Chem. Biol. 2019, 53, 44-50. [PubMed Link] [Publisher Link]
#Welsh, M. A.; #Schaefer, K.; Taguchi, A.; Kahne, D.; Walker, S. Direction of Chain Growth and Substrate Preferences of Shape, Elongation, Division, and Sporulation-Family Peptidoglycan Glycosyltransferases. J. Am. Chem. Soc. 2019, 141 (33), 12994-12997. [PubMed Link] [Publisher Link]
#Lane, E. A.; #Choi, D. W.; Garcia-Haro, L.; Levine, Z. G.; Tedoldi, M.; Walker, S.; Danial, N. N. HCF-1 Regulates De Novo Lipogenesis through a Nutrient-Sensitive Complex with ChREBP. Mol. Cell 2019, 25 (2), 357-371.e7. [PubMed Link] [Publisher Link]
Itkonen, H. M.; Poulose, N.; Walker, S.; Mills, I. G. CDK9 Inhibition Induces a Metabolic Switch that Renders Prostate Cancer Cells Dependent on Fatty Acid Oxidation. Neoplasia 2019, 21 (7), 713-720. [PubMed Link] [Publisher Link]
Itkonen, H. M.; Urbanucci, A.; Martin, S. E. S.; Khan, A.; Mathelier, A.; Thiede, B.; Walker, S.; Mills, I. G. High OGT activity is essential for MYC-driven proliferation of prostate cancer cells. Theranostics 2019, 9 (8), 2183-2197. [PubMed Link] [Publisher Link]
Taguchi, A.; Welsh, M. A.; Marmont, L. S.; Lee, W.; Sjodt, M.; Kruse, A. C.; Kahne, D.; *Bernhardt, T. G.; *Walker, S. FtsW is a peptidoglycan polymerase that is functional only in complex with its cognate penicillin-binding protein. Nat. Microbiol. 2019, 4, 587-594. [PubMed Link] [Publisher Link]
Joiner, C. M.; Li, H.; Jiang, J.; Walker, S. Structural characterization of the O-GlcNAc cycling enzymes: insights into substrate recognition and catalytic mechanisms. Curr. Opin. Struct. Biol. 2019, 56, 97-106. [PubMed Link] [Publisher Link]
Sehin, Y.; Koshla, O.; Dacyuk, Y.; Zhao, R.; Ross, R.; Myronovskyi, M.; Limbach, P. A.; Luzhetskyy, A.; Walker, S.; Fedorenko, V.; Ostash, B. Gene ssfg_01967 (miaB) for tRNA modification influences morphogenesis and moenomycin biosynthesis in Streptomyces ghanaensis ATCC14672. Microbiology 2019, 165 (2), 233-245. [PubMed Link] [Publisher Link]
2018
Wood, B. M.; Santa Maria, J. P.; Matano, L. M.; Vickery, C. R.; Walker, S. A partial reconstitution implicates DltD in catalyzing lipoteichoic acid D-alanylation. J. Biol. Chem. 2018, 293 (46), 17985-17996. [PubMed Link] [Publisher Link]
#Martin, S. E. S.; #Tan, Z.-W.; Itkonen, H. M.; Duveau, D. Y.; Paulo, J. A.; Janetzko, J.; Boutz, P. L.; Törk, L.; Moss, F. A.; Thomas, C. J.; Gygi, S. P.; Lazarus, M. B.; Walker, S. Structure-Based Evolution of Low Nanomolar O-GlcNAc Transferase Inhibitors. J. Am. Chem. Soc. 2018, 140 (42), 13542-13545. [PubMed Link] [Publisher Link]
Rohs, P. D. A.; Buss, J.; Sim, S. I.; Squyres, G. R.; Srisuknimit, V.; Smith, M.; Cho, H.; Sjodt, M.; Kruse, A. C.; Garner, E. C.; Walker, S.; Kahne, D. E.; Bernhardt, T. G. A central role for PBP2 in the activation of peptidoglycan polymerization by the bacterial cell elongation machinery. PLoS Genet. 2018, 14 (10), e1007726. [PubMed Link] [Publisher Link]
Baranowski, C.; Welsh, M. A.; Sham, L.-T.; Eskandarian, H. A.; Lim, H. C.; Kieser, K. J.; Wagner, J. C.; McKinney, J. D.; Fantner, G. E.; Ioerger, T. R.; Walker, S.; Bernhardt, T. G.; Rubin, E. J.; Rego, E. H. Maturing Mycobacterium smegmatis peptidoglycan reüluires non-canonical crosslinks to maintain shape. eLife 2018, 16 (7), e37516. [PubMed Link] [Publisher Link]
Buss, J. A.; Baidin, V.; Welsh, M. A.; Flores-Kim, J.; Cho, H.; Wood, B. M.; Uehara, T.; Walker, S.; Kahne, D. Bernhardt, T. G. Pathway-Directed Screen for Inhibitors of the Bacterial Cell Elongation Machinery. Antimicrob. Agents Chemother. 2018, 63 (1), e01530-18. [PubMed Link] [Publisher Link]
Lee, W.; Do, T.; Zhang, G.; Kahne, D.; Meredith, T. C.; Walker, S. Antibiotic Combinations That Enable One-Step, Targeted Mutagenesis of Chromosomal Genes. ACS Infect. Dis. 2018, 4 (6), 1007-1018. [PubMed Link] [Publisher Link]
Rubino, F. A.; Kumar, S.; Ruiz, N.; *Walker, S.; *Kahne, D. E. Membrane Potential Is Required for MurJ Function. J. Am. Chem. Soc. 2018, 140 (13), 4481-4484. [PubMed Link] [Publisher Link]
Sjodt, M.; Brock, K.; Dobihal, G.; Rohs, P. D. A.; Green, A. G.; Hopf, T. A.; Meeske, A. J.; Srisuknimit, V.; Kahne, D.; Walker, S.; Marks, D. S.; Bernhardt, T. G.; Rudner, D. Z.; Kruse, A. C. Structure of the peptidoglycan polymerase RodA resolved by evolutionary coupling analysis. Nature 2018, 556 (7699), 118-121. [PubMed Link] [Publisher Link]
Tiwari, K. B.; Gatto, C.; Walker, S.; Wilkinson, B. J. Exposure of Staphylococcus aureus to Targocil Blocks Translocation of the Major Autolysin Atl across the Membrane, Resulting in a Significant Decrease in Autolysis. Antimicrob. Agents Chemother. 2018, 62 (7), e00323-18. [PubMed Link] [Publisher Link]
#Santiago, M.; #Lee, W.; Fayad, A. A.; Coe, K. A.; Rajagopal, M.; Do, T.; Hennessen, F.; Srisuknimit, V.; Müller, R.; Meredith, T. C.; Walker, S. Genome-wide mutant profiling predicts the mechanism of a Lipid II binding antibiotic. Nat. Chem. Biol. 2018, 14 (6), 601-608. [PubMed Link] [Publisher Link]
Levine, Z. G.; Fan, C.; Melicher, M. S.; Orman, M.; Benjamin, T.; Walker, S. O-GlcNAc Transferase Recognizes Protein Substrates Using an Asparagine Ladder in the Tetratricopeptide Repeat (TPR) Superhelix. J. Am. Chem. Soc. 2018, 140 (10), 3510-3513. [PubMed Link] [Publisher Link]
#Hussain, S.; #Wivagg, C. N.; Szwedziak, P.; Wong, F.; Schaefer, K.; Izoré, T.; Renner, L. D.; Holmes, M. J.; Sun, Y.; Bisson-Filho, A. W.; Walker, S.; Amir, A.; Löwe, J.; Garner, E. C. MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis. eLife 2018, 22 (7), e32471. [PubMed Link] [Publisher Link]
#Schaefer, K.; #Owens, T. W.; Kahne, D.; Walker, S. Substrate Preferences Establish the Order of Cell Wall Assembly in Staphylococcus aureus. J. Am. Chem. Soc. 2018, 140 (7), 2442-2445. [PubMed Link] [Publisher Link]
Vickery, C. R.; Wood, B. M.; Morris, H. G.; Losick, R.; Walker, S. Reconstitution of Staphylococcus aureus Lipoteichoic Acid Synthase Activity Identifies Congo Red as a Selective Inhibitor. J. Am. Chem. Soc. 2018, 140 (3), 876-879. [PubMed Link] [Publisher Link]
2017
Welsh, M. A.; Taguchi, A.; Schaefer, K.; Van Tyne, D.; Lebreton, F.; Gilmore, M. S.; Kahne, D.; Walker, S. Identification of a Functionally Unique Family of Penicillin-Binding Proteins. J. Am. Chem. Soc. 2017, 139 (49), 17727-17730. [PubMed Link] [Publisher Link]
#Matano, L. M.; #Morris, H. G.; #Hesser, A. R.; Martin, S. E. S.; Lee, W.; Owens, T. W.; Laney, E.; Nakaminami, H.; Hooper, D.; Meredith, T. C.; Walker, S. Antibiotic That Inhibits the ATPase Activity of an ATP-Binding Cassette Transporter by Binding to a Remote Extracellular Site. J. Am. Chem. Soc. 2017, 139 (31), 10597-10600. [PubMed Link] [Publisher Link]
#DeFrancesco, A. S.; #Masloboeva, N.; Syed, A. K.; DeLoughery, A.; Bradshaw, N.; Li, G.-W.; Gilmore, M. S.; Walker, S.; Losick, R. Genome-wide screen for genes involved in eDNA release during biofilm formation by Staphylococcus aureus. Proc. Natl. Acad. Sci. U. S. A. 2017, 114 (29), E5969-E5978. [PubMed Link] [Publisher Link]
#Qiao, Y.; #Srisuknimit, V.; Rubino, F.; Schaefer, K.; Ruiz, N.; Walker, S.; Kahne, D. Lipid II overproduction allows direct assay of transpeptidase i, nhibition by β-lactams. Nat. Chem. Biol. 2017, 13 (7), 793-798. [PubMed Link] [Publisher Link]
#Srisuknimit, V.; #Qiao, Y.; Schaefer, K.; Kahne, D.; Walker, S. Peptidoglycan Cross-Linking Preferences of Staphylococcus aureus Penicillin-Binding Proteins Have Implications for Treating MRSA Infections. J. Am. Chem. Soc. 2017, 139 (29), 9791-9794. [PubMed Link] [Publisher Link]
Schaefer, K.; Matano, L. M.; Qiao, Y.; Kahne, D.; Walker, S. In vitro reconstitution demonstrates the cell wall ligase activity of LCP proteins. Nat. Chem. Biol. 2017, 13 (4), 396-401. [PubMed Link] [Publisher Link]
Janetzko, J.; Walker, S. Aspartate Glycosylation Triggers Isomerization to Isoaspartate. J. Am. Chem. Soc. 2017, 139 (9), 3332-3335. [PubMed Link] [Publisher Link]
Lee, W.; VanderVen, B. C.; Walker, S.; Russell, D. G. Novel protein acetyltransferase, Rv2170, modulates carbon and energy metabolism in Mycobacterium tuberculosis. Sci. Rep. 2017, 7 (1), 72. [PubMed Link] [Publisher Link]
Rajagopal, M.; Walker, S. Envelope Structures of Gram-Positive Bacteria. Curr. Top. Microbiol. Immunol. 2017, 404, 1-44. [PubMed Link] [Publisher Link]
2016
Matano, L. M.; Morris, H. G.; Wood, B. M.; Meredith, T. C.; Walker, S. Accelerating the discovery of antibacterial compounds using pathway-directed whole cell screening. Bioorg. Med. Chem. 2016, 24 (24), 6307-6314. [PubMed Link] [Publisher Link]
Janetzko, J.; Trauger, S. A.; Lazarus, M. B.; Walker, S. How the glycosyltransferase OGT catalyzes amide bond cleavage. Nat. Chem. Biol. 2016, 12 (11), 899-901. [PubMed Link] [Publisher Link]
#Rajagopal, M.; #Martin, M. J.; #Santiago, M.; Lee, W.; Kos, V. N.; Meredith, T.; Gilmore, M. S.; Walker, S. Multidrug Instrinsic Resistance Factors in Staphylococcus aureus Identified by Profiling Fitness within High-Diversity Transposon Libraries. mBio 2016, 7 (4), e00950-16. [PubMed Link] [Publisher Link]
Meeske, A. J.; Riley, E. P.; Robins, W. P.; Uehara, T.; Mekalanos, J. J.; Kahne, D.; Walker, S.; Kruse, A. C.; Bernhardt, T. G.; Rudner, D. Z. SEDS proteins are a widespread family of bacterial cell wall polymerases. Nature 2016, 537 (7622), 634-638. [PubMed Link] [Publisher Link]
Levine, Z. G.; Walker, S. The Biochemistry of O-GlcNAc Transferase: Which Functions Make It Essential in Mammalian Cells? Annu. Rev. Biochem. 2016, 85, 631-657. [PubMed Link] [Publisher Link]
#Horbal, L.; #Ostash, B.; Luzhetskyy, A.; Walker, S.; Kalinowski, J.; Fedorenko, V. A gene cluster for the biosynthesis of moenomycin family antibiotics in the genome of teicoplanin producer Actinoplanes teichomyceticus. Appl. Microbiol. Biotechnol. 2016, 100 (17), 7629-7638. [PubMed Link] [Publisher Link]
Markovski, M.; Bohrhunter, J. L.; Lupoli, T. J.; Uehara, T.; Walker, S.; Kahne, D. E.; Bernhardt, T. G. Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity. Proc. Natl. Acad. Sci. U. S. A. 2016, 113 (17), 4788-4793. [PubMed Link] [Publisher Link]
Goodreid, J. D.; Janetzko, J.; Santa Maria, J. P.; Wong, K. S.; Leung, E.; Eger, B. T.; Bryson, S.; Pai, E. F.; Gray-Owen, S. D.; Walker, S.; Houry, W. A.; Batey, R. A. Development and Characterization of Potent Cyclic Acyldepsipeptide Analogues with Increased Antimicrobial Activity. J. Med. Chem. 2016, 59 (2), 624-646. [PubMed Link] [Publisher Link]
Lee, W.; Schaefer, K.; Qiao, Y.; Srisuknimit, V.; Steinmetz, H.; Müller, R.; Kahne, D.; Walker, S. The Mechanism of Action of Lysobactin. J. Am. Chem. Soc. 2016, 138 (1), 100-103. [PubMed Link] [Publisher Link]
Pasquina, L.; Santa Maria, J. P.; Wood, B. M.; Moussa, S. H.; Matano, L. M.; Santiago, M.; Martin, S. E. S.; Lee, W.; Meredith, T. C.; Walker, S. A synthetic lethal approach for compound and target identification in Staphylococcus aureus. Nat. Chem. Biol. 2016, 12 (1), 40-45. [PubMed Link] [Publisher Link]
2015
Angelova, M.; Ortiz-Meoz, R. F.; Walker, S.; Knipe, D. M. Inhibition of O-Linked N-Acetylglucosamine Transferase Reduces Replication of Herpes Simplex Virus and Human Cytomegalovirus. J. Virol. 2015, 89 (16), 8474-8483. [PubMed Link] [Publisher Link]
Santiago, M.; Matano, L. M.; Moussa, S. H.; Gilmore, M. S.; Walker, S.; Meredith, T. C. A new platform for ultra-high density Staphylococcus aureus transposon libraries. BMC Genomics 2015, 16, 252. [PubMed Link] [Publisher Link]
Ortiz-Meoz, R. F.; Jiang, J.; Lazarus, M. B.; Orman, M.; Janetzko, J.; Fan, C.; Duveau, D. Y.; Tan, Z.-W.; Thomas, C. J.; Walker, S. A Small Molecule That Inhibits OGT Activity in Cells. ACS Chem. Biol. 2015, 10 (6), 1392-1397. [PubMed Link] [Publisher Link]
Schirner, K.; Eun, Y.-J.; Dion, M.; Luo, Y.; Helmann, J. D.; Garner, E. C.; Walker, S. Lipid-linked cell wall precursors regulate membrane association of bacterial actin MreB. Nat. Chem. Biol. 2015, 11 (1), 38-45. [PubMed Link] [Publisher Link]
2014
Qiao, Y.; Lebar, M. D.; Schirner, K.; Schaefer, K.; Tsukamoto, H.; Kahne, D.; Walker, S. Detection of Lipid-Linked Peptidoglycan Precursors by Exploiting an Unexpected Transpeptidase Reaction. J. Am. Chem. Soc. 2014, 136 (42), 14678-14681. [PubMed Link] [Publisher Link]
Janetzko, J.; Walker, S. The Making of a Sweet Modification: Structure and Function of O-GlcNAc Transferase. J. Biol. Chem. 2014, 289 (50), 34424-24432. [PubMed Link] [Publisher Link]
#Valentino, M. D.; #Foulston, L.; #Sadaka, A.; Kos, V. N.; Villet, R. A.; Santa Maria, J.; Lazinski, D. W.; Camilli, A.; Walker, S.; Hooper, D. C.; Gilmore, M. S. Genes Contributing to Staphylococcus aureus Fitness in Abscess- and Infection-Related Ecologies. mBio 2014, 5 (5), e01729-14. [PubMed Link] [Publisher Link]
Santa Maria, J. P.; Sadaka, A.; Moussa, S. H.; Brown, S.; Zhang, Y. J.; Rubin, E. J.; Gilmore, M. S.; Walker, S. Compound-gene interaction mapping reveals distinct roles for Staphylococcus aureus teichoic acids. Proc. Natl. Acad. Sci. U. S. A. 2014, 111 (34), 12510-12515. [PubMed Link] [Publisher Link]
#Lebar, M. D.; #May, J. M.; Meeske, A. J.; Leiman, S. A.; Lupoli, T. J.; Tsukamoto, H.; Losick, R.; Rudner, D. Z.; Walker, S.; Kahne, D. Reconstitution of Peptidoglycan Cross-Linking Leads to Improved Fluorescent Probes of Cell Wall Synthesis. J. Am. Chem. Soc. 2014, 136 (31), 10874-10877. [PubMed Link] [Publisher Link]
Sherman, D. J.; Lazarus, M. B.; Murphy, L.; Liu, C.; Walker, S.; Ruiz, N.; Kahne, D. Decoupling catalytic activity from biological function of the ATPase that powers lipopolysaccharide transport. Proc. Natl. Acad. Sci. U. S. A. 2014, 111 (13), 4982-4987. [PubMed Link] [Publisher Link]
Ortiz-Meoz, R. F.; Merbl, Y.; Kirschner, M. W.; Walker, S. Microarray Discovery of New OGT Substrates: The Medulloblastoma Oncogene OTX2 Is O-GlcNAcylated. J. Am. Chem. Soc. 2014, 136 (13), 4845-4848. [PubMed Link] [Publisher Link]
Lupoli, T. J.; Lebar, M. D.; Markovski, M.; Bernhardt, T.; Kahne, D.; Walker, S. Lipoprotein Activators Stimulate Escherichia coli Penicillin-Binding Proteins by Different Mechanisms. J. Am. Chem. Soc. 2014, 136 (1), 52-55. [PubMed Link] [Publisher Link]
Rebets, Y.; Lupoli, T.; Qiao, Y.; Schirner, K.; Villet, R.; Hooper, D.; Kahne, D.; Walker, S. Moenomycin Resistance Mutations in Staphylococcus aureus Reduce Peptidoglycan Chain Length and Cause Aberrant Cell Division. ACS Chem. Biol. 2014, 9 (2), 459-467. [PubMed Link] [Publisher Link]
2013
Makitrynskyy, R.; Ostash, B.; Tsypik, O.; Rebets, Y.; Doud, E.; Meredith, T.; Luzhetskyy, A.; Bechthold, A.; Walker, S.; Fedorenko, V. Pleiotropic regulatory genes bldA, adpA and absB are implicated in production of phosphoglycolipid antibiotic moenomycin. Open Biol. 2013, 3 (10), 130121. [PubMed Link] [Publisher Link]
Wang, H.; Gill, C. J.; Lee, S. H.; Mann, P.; Zuck, P.; Meredith, T. C.; Murgolo, N.; She, X.; Kales, S.; Liang, L.; Liu, J.; Wu, J.; Santa Maria, J.; Su, J.; Pan, J.; Hailey, J.; Mcguinness, D.; Tan, C. M.; Flattery, A.; Walker, S.; Black, T.; Roemer, T. Discovery of Wall Teichoic Acid Inhibitors as Potential Anti-MRSA β-Lactam Combination Agents. Chem. Biol. 2013, 20 (2), 272-284. [PubMed Link] [Publisher Link]
Lebar, M. D.; Lupoli, T. J.; Tsukamoto, H.; May, J. M.; Walker, S.; Kahne, D. Forming Cross-Linked Peptidoglycan from Synthetic Gram-Negative Lipid II. J. Am. Chem. Soc. 2013, 135 (12), 4632-4635. [PubMed Link] [Publisher Link]
#Lazarus, M. B.; #Jiang, J.; Kapuria, V.; Bhuiyan, T.; Janetzko, J.; Zandberg, W. F.; Vocadlo, D. J.; Herr, W.; Walker, S. HCF-1 Is Cleaved in the Active Site of O-GlcNAc Transferase. Science 2013, 342 (6163), 1235-1239. [PubMed Link] [Publisher Link]
Garg, N.; Oman, T. J.; Wang, T.-S. A.; De Gonzalo, C. V. G.; Walker, S.; van der Donk, W. A. Mode of action and structure-activity relationship studies of geobacillin I. J. Antibiot. (Tokyo) 2013, 67 (1), 133-136. [PubMed Link] [Publisher Link]
Ostash, B.; Campbell, J.; Luzhetskyy, A.; Walker, S. MoeH5: a natural glycorandomizer from the moenomycin biosynthetic pathway. Mol. Microbiol. 2013, 90 (6), 1324-1338. [PubMed Link] [Publisher Link]
Pasquina, L. W.; Santa Maria, J. P.; Walker, S. Teichoic acid biosynthesis as an antibiotic target. Curr. Opin. Microbiol. 2013, 16 (5), 531-537. [PubMed Link] [Publisher Link]
Gampe, C. M.; Tsukamoto, H.; Doud, E. H.; Walker, S.; Kahne, D. Tuning the Moenomycin Pharmacophore To Enable Discovery of Bacterial Cell Wall Synthesis Inhibitors. J. Am. Chem. Soc. 2013, 135 (10), 3776-3779. [PubMed Link] [Publisher Link]
Brown, S.; Santa Maria, J. P.; Walker, S. Wall Teichoic Acids of Gram-Positive Bacteria. Annu. Rev. Microbiol. 2013, 67, 313-336. [PubMed Link] [Publisher Link]
2012
#Jiang, J.; #Lazarus, M. B.; Pasquina, L.; Sliz, P.; Walker, S. A neutral diphosphate mimic crosslinks the active site of human O-GlcNAc transferase. Nat. Chem. Biol. 2012, 8 (1), 72-77. [PubMed Link] [Publisher Link]
Palmer, K. L.; Godfrey, P.; Griggs, A.; Kos, V. N.; Zucker, J.; Desjardins, C.; Cerqueira, G.; Gevers, D.; Walker, S.; Wortman, J.; Feldgarden, M.; Haas, B.; Birren, B.; Gilmore, M. S. Comparative Genomics of Enterococci: Variation in Enterococcus faecalis, Clade Structure in E. faecium, and Defining Characteristics of E. gallinarum and E. casseliflavus. mBio 2012, 3 (1), e00318-11. [PubMed Link] [Publisher Link]
#Knerr, P. J.; #Oman, T. J.; De Gonzalo, C. V. G.; Lupoli, T. J.; Walker, S.; van der Donk, W. A. Non-proteinogenic Amino Acids in Lacticin 481 Analogues Result in More Potent Inhibition of Peptidoglycan Transglycosylation. ACS Chem. Biol. 2012, 7 (11), 1791-1795. [PubMed Link] [Publisher Link]
#Lazarus, M. B.; #Jiang, J.; Gloster, T. M.; Zandberg, W. F.; Whitworth, G. E.; Vocadlo, D. J.; Walker, S. Structural snapshots of the reaction coordinate for O-GlcNAc transferase. Nat. Chem. Biol. 2012, 8 (12), 966-968. [PubMed Link] [Publisher Link]
*Ostash, B.; Doud, E.; Walker, S. ABC transporter genes from Streptomyces ghanaensis moenomycin biosynthetic gene cluster: roles in antibiotic production and export. Arch. Microbiol. 2012, 194 (11), 915-922. [PubMed Link] [Publisher Link]
#Campbell, J.; #Singh, A. K.; Swoboda, J. G.; Gilmore, M. S.; Wilkinson, B. J.; Walker, S. An Antibiotic That Inhibits a Late Step in Wall Teichoic Acid Biosynthesis Induces the Cell Wall Stress Stimulon in Staphylococcus aureus. Antimicrob. Agents Chemother. 2012, 56 (4), 1810-1820. [PubMed Link] [Publisher Link]
Brown, S.; Xia, G.; Luhachack, L. G.; Campbell, J.; Meredith, T. C.; Chen, C.; Winstel, V.; Gekeler, C.; Irazoqui, J. E.; Peschel, A.; Walker, S. Methicillin resistance in Staphylococcus aureus requires glycosylated wall teichoic acids. Proc. Natl. Acad. Sci. U. S. A. 2012, 109 (46), 18909-18914. [PubMed Link] [Publisher Link]
Suzuki, T.; Campbell, J.; Kim, Y.; Swoboda, J. G.; Mylonakis, E.; Walker, S.; Gilmore, M. S. Wall teichoic acid protects Staphylococcus aureus from inhibition by Congo red and other dyes. J. Antimicrob. Chemother. 2012, 67 (9), 2143-2151. [PubMed Link] [Publisher Link]
2011
Suzuki, T.; Swoboda, J. G.; Campbell, J.; Walker, S.; Gilmore, M. S. In vitro Antimicrobial Activity of Wall Teichoic Acid Biosynthesis Inhibitors against Staphylococcus aureus Isolates. Antimicrob. Agents Chemother. 2011, 55 (2), 767-774. [PubMed Link] [Publisher Link]
Campbell, J.; Singh, A. K.; Santa Maria, J. P.; Kim, Y.; Brown, S.; Swoboda, J. G.; Mylonakis, E.; Wilkinson, B. J.; Walker, S. Synthetic Lethal Compound Combinations Reveal a Fundamental Connection between Wall Teichoic Acid and Peptidoglycan Biosynthesis in Staphylococcus aureus. ACS Chem. Biol. 2011, 6 (1), 106-116. [PubMed Link] [Publisher Link]
Schirner, K.; Stone, L. K.; Walker, S. ABC Transporters Required for Export of Wall Teichoic Acids Do Not Discriminate between Different Main Chain Polymers. ACS Chem. Biol. 2011, 6 (5), 407-412. [PubMed Link] [Publisher Link]
#Ostash, B.; #Rebets, Y.; Myronovskyy, M.; Tsypik, O.; Ostash, I.; Kulachkovskyy, O.; Datsyuk, Y.; Nakamura, T.; Walker, S.; Fedorenko, V. Identification and characterization of the Streptomyces globisporus 1912 regulatory gene lndYR that affects sporulation and antibiotic production. Microbiology 2011, 157 (4), 1240-1249. [PubMed Link] [Publisher Link]
Suzuki, T.; Campbell, J.; Swoboda, J. G.; Walker, S.; Gilmore, M. S. Role of Wall Teichoic Acids in Staphylococcus aureus Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2011, 52 (6), 3187-3192. [PubMed Link] [Publisher Link]
Rabyk, M.; Ostash, B.; Rebets, Y.; Walker, S.; Fedorenko, V. Streptomyces ghanaensis pleiotropic regulatory gene wblA(gh) influences morphogenesis and moenomycin production. Biotechnol. Lett. 2011, 33 (12), 2481-2486. [PubMed Link] [Publisher Link]
#Lazarus, M. B.; #Nam, Y.; Jiang, J.; Sliz, P.; Walker, S. Structure of human O-GlcNAc transferase and its complex with a peptide substrate. Nature 2011, 469 (7331), 564-567. [PubMed Link] [Publisher Link]
Doud, E. H.; Perlstein, D. L.; Wolpert, M.; Cane, D. E.; Walker, S. Two Distinct Mechanisms for TIM Barrel Prenyltransferases in Bacteria. J. Am. Chem. Soc. 2011, 133 (5), 1270-1273. [PubMed Link] [Publisher Link]
Oman, T. J.; Lupoli, T. J.; Wang, T.-S. A.; Kahne, D.; Walker, S.; van der Donk, W. A. Haloduracin α Binds the Peptidoglycan Precursor Lipid II with 2:1 Stoichiometry. J. Am. Chem. Soc. 2011, 133 (44), 17544-17547. [PubMed Link] [Publisher Link]
Gampe, C. M.; Tsukamoto, H.; Wang, T.-S. A.; Walker, S.; Kahne, D. Modular synthesis of diphospholipid oligosaccharide fragments of the bacterial cell wall and their use to study the mechanism of moenomycin and other antibiotics. Tetrahedron 2011, 67 (51), 9771-9778. [PubMed Link] [Publisher Link]
Wang, T.-S. A.; Lupoli, T. J.; Sumida, Y.; Tsukamoto, H.; Wu, Y.; Rebets, Y.; Kahne, D. E.; Walker, S. Primer Preactivation of Peptidoglycan Polymerases. J. Am. Chem. Soc. 2011, 133 (22), 8528-8530. [PubMed Link] [Publisher Link]
Lupoli, T. J.; Tsukamoto, H.; Doud, E. H.; Wang, T.-S. A.; Walker, S.; Kahne, D. Transpeptidase-Mediated Incorporation of D-Amino Acids into Bacterial Peptidoglycan. J. Am. Chem. Soc. 2011, 133 (28), 10748-10751. [PubMed Link] [Publisher Link]
2010
#Caldwell, S. A.; #Jackson, S. R.; #Shahriari, K. S.; Lynch, T. P.; Sethi, G.; Walker, S.; Vosseller, K.; Reginato, M. J. Nutrient sensor O-GlcNAc transferase regulates breast cancer tumorigenesis through targeting of the oncogenic transcription factor FoxM1. Oncogene 2010, 29 (19), 2831-2842. [PubMed Link] [Publisher Link]
Swoboda, J. G.; Campbell, J.; Meredith, T. C.; Walker, S. Wall Teichoic Acid Function, Biosynthesis, and Inhibition. Chembiochem 2010, 11 (1), 35-45. [PubMed Link] [Publisher Link]
Brown, S.; Meredith, T.; Swoboda, J.; Walker, S. Staphylococcus aureus and Bacillus subtilis W23 Make Polyribitol Wall Teichoic Acids Using Different Enzymatic Pathways. Chem. Biol. 2010, 17 (10), 1101-1110. [PubMed Link] [Publisher Link]
Lee, K.; Campbell, J.; Swoboda, J. G.; Cuny, G. D.; Walker, S. Development of improved inhibitors of wall teichoic acid biosynthesis with potent activity against Staphylococcus aureus. Bioorg. Med. Chem. Lett. 2010, 20 (5), 1767-1770. [PubMed Link] [Publisher Link]
Makitrynskyy, R.; Rebets, Y.; Ostash, B.; Zaburannyi, N.; Rabyk, M.; Walker, S.; Fedorenko, V. Genetic factors that influence moenomycin production in streptomycetes. J. Ind. Microbiol. Biotechnol. 2010, 37 (6), 559-566. [PubMed Link] [Publisher Link]
Ostash, B.; Walker, S. Moenomycin family antibiotics: chemical synthesis, biosynthesis, and biological activity. Nat. Prod. Rep. 2010, 27 (11), 1594-1617. [PubMed Link] [Publisher Link]
Silhavy, T. J.; Kahne, D.; Walker, S. The Bacterial Cell Envelope. Cold Spring Harb. Perspect. Biol. 2010, 2 (5), a000414. [PubMed Link] [Publisher Link]
Fuse, S.; Tsukamoto, H.; Yuan, Y.; Wang, T.-S. A.; Zhang, Y.; Bolla, M.; Walker, S.; Sliz, P.; Kahne, D. Functional and Structural Analysis of a Key Region of the Cell Wall Inhibitor Moenomycin. ACS Chem. Biol. 2010, 5 (7), 701-711. [PubMed Link] [Publisher Link]
#Paradis-Bleau, C.; #Markovski, M.; #Uehara, T.; Lupoli, T. J.; Walker, S.; Kahne, D. E.; Bernhardt, T. G. Lipoprotein Cofactors Located in the Outer Membrane Activate Bacterial Cell Wall Polymerases. Cell 2010, 143 (7), 1110-1120. [PubMed Link] [Publisher Link]
Perlstein, D. L.; Wang, T.-S. A.; Doud, E. H.; Kahne, D.; Walker, S. The Role of the Substrate Lipid in Processive Glycan Polymerization by the Peptidoglycan Glycosyltransferases. J. Am. Chem. Soc. 2010, 132 (1), 48-49. [PubMed Link] [Publisher Link]
#Shi, Y.; #Tomic, J.; Wen, F.; Shaha, S.; Bahlo, A.; Harrison, R.; Dennis, J. W.; Williams, R.; Gross, B. J.; Walker, S.; Zuccolo, J.; Deans, J. P.; Hart, G. W.; Spaner, D. E. Aberrant O-GlcNAcylation characterizes chronic lymphocytic leukemia. Leukemia 2010, 24 (9), 1588-1598. [PubMed Link] [Publisher Link]
Ostash, B.; Ostash, I.; Zhu, L.; Kharel, M. K.; Luzhetskyy, A.; Bechthold, A.; Walker, S.; Rohr, J.; Fedorenko, V. Properties of lanK-based regulatory circuit involved in landomycin biosynthesis in Streptomyces cyanogenus S136. Genetika 2010, 46 (5), 604-609. [PubMed Link] [Publisher Link]
2009
Swoboda, J. G.; Meredith, T. C.; Campbell, J.; Brown, S.; Suzuki, T.; Bollenbach, T.; Malhowski, A. J.; Kishony, R.; Gilmore, M. S.; Walker, S. Discovery of a Small Molecule that Blocks Wall Teichoic Acid Biosynthesis in Staphylococcus aureus. ACS Chem. Biol. 2009, 4 (10), 875-883. [PubMed Link] [Publisher Link]
Fang, X.; Nam, J.; Shin, D.; Rew, Y.; Boger, D. L.; Walker, S. Functional and biochemical analysis of a key series of ramoplanin analogues. Bioorg. Med. Chem. Lett. 2009, 19 (21), 6189-6191. [PubMed Link] [Publisher Link]
Ostash, B.; Makitrinskyy, R.; Walker, S.; Fedorenko, V. Identification and characterization of Streptomyces ghanaensis ATCC14672 integration sites for three actinophage-based plasmids. Plasmid 2009, 61 (3), 171-175. [PubMed Link] [Publisher Link]
Ostash, B.; Doud, E. H.; Lin, C.; Ostash, I.; Perlstein, D. L.; Fuse, S.; Wolpert, M.; Kahne, D.; Walker, S. Complete Characterization of the Seventeen Step Moenomycin Biosynthetic Pathway. Biochemistry 2009, 48 (37), 8830-8841. [PubMed Link] [Publisher Link]
Lupoli, T. J.; Taniguchi, T.; Wang, T.-S.; Perlstein, D. L.; Walker, S.; Kahne, D. E. Studying a Cell Division Amidase Using Defined Peptidoglycan Substrates. J. Am. Chem. Soc. 2009, 131 (51), 18230-18231. [PubMed Link] [Publisher Link]
2008
Ostash, I.; Rebets, Y.; Ostash, B.; Kobylyanskyy, A.; Myronovskyy, M.; Nakamura, T.; Walker, S.; Fedorenko, V. An ABC transporter encoding gene lndW confers resistance to landomycin E. Arch. Microbiol. 2008, 190 (1), 105-109. [PubMed Link] [Publisher Link]
Ostash, I.; Ostash, B.; Luzhetskyy, A.; Bechthold, A.; Walker, S.; Fedorenko, V. Coordination of export and glycosylation of landomycins in Streptomyces cyanogenus S136. FEMS Microbiol. Lett. 2008, 285 (2), 195-202. [PubMed Link] [Publisher Link]
Meredith, T. C.; Swoboda, J. G.; Walker, S. Late-Stage Polyribitol Phosphate Wall Teichoic Acid Biosynthesis in Staphylococcus aureus. J. Bacteriol. 2008, 190 (8), 3046-3056. [PubMed Link] [Publisher Link]
Brown, S.; Zhang, Y.-H.; Walker, S. A Revised Pathway Proposed for Staphylococcus aureus Wall Teichoic Acid Biosynthesis Based on In Vitro Reconstitution of the Intracellular Steps. Chem. Biol. 2008, 15 (1), 12-21. [PubMed Link] [Publisher Link]
Gross, B. J.; Swoboda, J. G.; Walker, S. A Strategy to Discover Inhibitors of O-Linked Glycosylation. J. Am. Chem. Soc. 2008, 130 (2), 440-441. [PubMed Link] [Publisher Link]
Wang, T.-S. A.; Manning, S. A.; Walker, S.; Kahne, D. Isolated Peptidoglycan Glycosyltransferases from Different Organisms Produce Different Glycan Chain Lengths. J. Am. Chem. Soc. 2008, 130 (43), 14068-14069. [PubMed Link] [Publisher Link]
Yuan, Y.; Fuse, S.; Ostash, B.; Sliz, P.; Kahne, D.; Walker, S. Structural Analysis of the Contacts Anchoring Moenomycin to Peptidoglycan Glycosyltransferases and Implications for Antibiotic Design. ACS Chem. Biol. 2008, 3 (7), 429-436. [PubMed Link] [Publisher Link]
2007
Dehennaut, V.; Lefebvre, T.; Sellier, C.; Leroy, Y.; Gross, B.; Walker, S.; Cacan, R.; Michalski, J.-C.; Vilain, J.-P.; Bodart, J.-F. O-Linked N-Acetylglucosaminyltransferase Inhibition Prevents G(2)/M Transition in Xenopus laevis Oocytes. J. Biol. Chem. 2007, 282 (17), 12527-12536. [PubMed Link] [Publisher Link]
Ostash, B.; Saghatelian, A.; Walker, S. A Streamlined Metabolic Pathway for the Biosynthesis of Moenomycin A. Chem. Biol. 2007, 14 (3), 257-267. [PubMed Link] [Publisher Link]
Barrett, D.; Wang, T.-S. A.; Yuan, Y.; Zhang, Y.; Kahne, D.; Walker, S. Analysis of Glycan Polymers Produced by Peptidoglycan Glycosyltransferases. J. Biol. Chem. 2007, 282 (44), 31964-31971. [PubMed Link] [Publisher Link]
Leimkuhler, C.; Fridman, M.; Lupoli, T.; Walker, S.; Walsh, C. T.; Kahne, D. Characterization of Rhodosaminyl Transfer by the AknS/AknT Glycosylation Complex and Its Use in Reconstituting the Biosynthetic Pathway of Aclacinomycin A. J. Am. Chem. Soc. 2007, 129 (34), 10546-10550. [PubMed Link] [Publisher Link]
Yuan, Y.; Barrett, D.; Zhang, Y.; Kahne, D.; Sliz, P.; Walker, S. Crystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis. Proc. Natl. Acad. Sci. U. S. A. 2007, 104 (13), 5348-5353. [PubMed Link] [Publisher Link]
Perlstein, D. L.; Zhang, Y.; Wang, T.-S.; Kahne, D. E.; Walker, S. The Direction of Glycan Chain Elongation by Peptidoglycan Glycosyltransferases. J. Am. Chem. Soc. 2007, 129 (42), 12674-12675. [PubMed Link] [Publisher Link]
Zhang, Y.; Fechter, E. J.; Wang, T.-S. A.; Barrett, D.; Walker, S.; Kahne, D. E. Synthesis of Heptaprenyl-Lipid IV to Analyze Peptidoglycan Glycosyltransferases. J. Am. Chem. Soc. 2007, 129 (11), 3080-3081. [PubMed Link] [Publisher Link]
Ostash, I.; Ostash, B.; Walker, S.; Fedorenko, V. Proton-dependent transporter gene lndJ confers resistance to landomycin E in Streptomyces globisporus 1912. Genetika 2007, 43 (8), 1032-1037. [PubMed Link] [Publisher Link]
2006
Zhang, Y.-H.; Ginsberg, C.; Yuan, Y.; Walker, S. Acceptor Substrate Selectivity and Kinetic Mechanism of Bacillus subtilis TagA. Biochemistry 2006, 45 (36), 10895-10904. [PubMed Link] [Publisher Link]
Love, K. R.; Swoboda, J. G.; Noren, C. J.; Walker, S. Enabling Glycosyltransferase Evolution: A Facile Substrate-Attachment Strategy for Phage-Display Enzyme Evolution. Chembiochem 2006, 7 (5), 753-756. [PubMed Link] [Publisher Link]
Tiyanont, K.; Doan, T.; Lazarus, M. B.; Fang, X.; Rudner, D. Z.; Walker, S. Imaging peptidoglycan biosynthesis in Bacillus subtilis with fluorescent antibiotics. Proc. Natl. Acad. Sci. U. S. A. 2006, 103 (29), 11033-11038. [PubMed Link] [Publisher Link]
Ginsberg, C.; Zhang, Y.-H.; Yuan, Y.; Walker, S. In Vitro Reconstitution of Two Essential Steps in Wall Teichoic Acid Biosynthesis. ACS Chem. Biol. 2006, 1 (1), 25-28. [PubMed Link] [Publisher Link]
Fang, X.; Tiyanont, K.; Zhang, Y.; Wanner, J.; Boger, D.; Walker, S. The mechanism of action of ramoplanin and enduracin. Mol. Biosyst. 2006, 2 (1), 69-76. [PubMed Link] [Publisher Link]
2005
Ostash, B.; Walker, S. Bacterial transglycosylase inhibitors. Curr. Opin. Chem. Biol. 2005, 9 (5), 459-466. [PubMed Link] [Publisher Link]
Walker, S.; Chen, L.; Hu, Y.; Rew, Y.; Shin, D.; Boger, D. L. Chemistry and Biology of Ramoplanin: A Lipoglycodepsipeptide with Potent Antibiotic Activity. Chem. Rev. 2005, 105 (2), 449-476. [PubMed Link] [Publisher Link]
Gross, B. J.; Kraybill, B. C.; Walker, S. Discovery of O-GlcNAc Transferase Inhibitors. J. Am. Chem. Soc. 2005, 127 (42), 14588-14589. [PubMed Link] [Publisher Link]
Barrett, D.; Leimkuhler, C.; Chen, L.; Walker, D.; Kahne, D.; Walker, S. Kinetic Characterization of the Glycosyltransferase Module of Staphylococcus aureus PBP2. J. Bacteriol. 2005, 187 (6), 2215-2217. [PubMed Link] [Publisher Link]
Leimkuhler, C.; Chen, L.; Barrett, D.; Panzone, G.; Sun, B.; Falcone, B.; Oberthür, M.; Donadio, S.; Walker, S.; Kahne, D. Differential Inhibition of Staphylococcus aureus PBP2 by Glycopeptide Antibiotics. J. Am. Chem. Soc. 2005, 127 (10), 3250-3251. [PubMed Link] [Publisher Link]
Yuan, Y.; Chung, H. S.; Leimkuhler, C.; Walsh, C. T.; Kahne, D.; Walker, S. In Vitro Reconstitution of EryCIII Activity for the Preparation of Unnatural Macrolides. J. Am. Chem. Soc. 2005, 127 (41), 14128-14129. [PubMed Link] [Publisher Link]
2004
Chen, L.; Yuan, Y.; Helm, J. S.; Hu, Y.; Rew, Y.; Shin, D.; Boger, D. L.; Walker, S. Dissecting Ramoplanin: Mechanistic Analysis of Synthetic Ramoplanin Analogues as a Guide to the Design of Improved Antibiotics. J. Am. Chem. Soc. 2004, 126 (24), 7462-7463. [PubMed Link] [Publisher Link]
Barrett, D. S.; Chen, L.; Litterman, N. K.; Walker, S. Expression and Characterization of the Isolated Glycosyltransferase Module of Escherichia coli PBP1b. Biochemistry 2004, 43 (38), 12375-12381. [PubMed Link] [Publisher Link]
Hu, Y.; Helm, J. S.; Chen, L.; Ginsberg, C.; Gross, B.; Kraybill, B.; Tiyanont, K.; Fang, X.; Wu, T.; Walker, S. Identification of Selective Inhibitors for the Glycosyltransferase MurG via High-Throughput Screening. Cell Chem. Biol. 2004, 11 (5), 703-711. [PubMed Link] [Publisher Link]
Garneau, S.; Qiao, L.; Chen, L.; Walker, S.; Vederas, J. C. Synthesis of mono- and disaccharide analogs of moenomycin and lipid II for inhibition of transglycosylase activity of penicillin-binding protein 1b. Bioorg. Med. Chem. 2004, 12 (24), 6473-6494. [PubMed Link] [Publisher Link]
Lee, H. Y.; Chung, H. S.; Hang, C.; Khosla, C.; Walsh, C. T.; Kahne, D.; Walker, S. Reconstitution and Characterization of a New Desosaminyl Transferase, EryCIII, from the Erythromycin Biosynthetic Pathway. J. Am. Chem. Soc. 2004, 126 (32), 9924-9925. [PubMed Link] [Publisher Link]
2003
Hu, Y.; Chen, L.; Ha, S.; Gross, B.; Falcone, B.; Walker, D.; Mokhtarzadeh, M.; Walker, S. Crystal structure of the MurG:UDP-GlcNAc complex reveals common structural principles of a superfamily of glycosyltransferases. Proc. Natl. Acad. Sci. U. S. A. 2003, 100 (3), 845-859. [PubMed Link] [Publisher Link]
Breukink, E.; van Heusden, H. E.; Vollmerhaus, P. J.; Swiezewska, E.; Brunner, L.; Walker, S.; Heck, A. J. R.; de Kruijff, B. Lipid II Is an Intrinsic Component of the Pore Induced by Nisin in Bacterial Membranes. J. Biol. Chem. 2003, 278 (22), 19898-19903. [PubMed Link] [Publisher Link]
Hu, Y.; Helm, J. S.; Chen, L.; Ye, X.-Y.; Walker, S. Ramoplanin Inhibits Bacterial Transglycosylases by Binding as a Dimer to Lipid II. J. Am. Chem. Soc. 2003, 125 (29), 8736-8737. [PubMed Link] [Publisher Link]
Chen, L.; Walker, D.; Sun, B.; Hu, Y.; Walker, S.; Kahne, D. Vancomycin analogues active against vanA-resistant strains inhibit bacterial transglycosylase without binding substrate. Proc. Natl. Acad. Sci. U. S. A. 2003, 100 (10), 5658-5663. [PubMed Link] [Publisher Link]
Helm, J. S.; Hu, Y.; Chen, L.; Gross, B.; Walker, S. Identification of Active-Site Inhibitors of MurG Using a Generalizable, High-Throughput Glycosyltransferase Screen. J. Am. Chem. Soc. 2003, 125 (37), 11168-11169. [PubMed Link] [Publisher Link]
2002
Chen, L.; Men, H.; Ha, S.; Ye, X.-Y.; Brunner, L.; Hu, Y.; Walker, S. Intrinsic Lipid Preferences and Kinetic Mechanism of Escherichia coli MurG. Biochemistry 2002, 41 (21), 6824-6833. [PubMed Link] [Publisher Link]
Hu, Y.; Walker, S. Remarkable Structural Similarities between Diverse Glycosyltransferases. Cell Chem. Biol. 2002, 9 (12), 1287-1296. [PubMed Link] [Publisher Link]
Helm, J. S.; Chen, L.; Walker, S. Rethinking Ramoplanin: The Role of Substrate Binding in Inhibition of Peptidoglycan Biosynthesis. J. Am. Chem. Soc. 2002, 124 (47), 13970-13971. [PubMed Link] [Publisher Link]
Lazar, K.; Walker, S. Substrate analogues to study cell-wall biosynthesis and its inhibition. Curr. Opin. Chem. Biol. 2002, 6 (6), 786-793. [PubMed Link] [Publisher Link]
2001
Ye, X.-Y.; Lo, M.-C.; Brunner, L.; Walker, D.; Kahne, D.; Walker, S. Better Substrates for Bacterial Transglycosylases. J. Am. Chem. Soc. 2001, 123 (13), 3155-3156. [PubMed Link] [Publisher Link]
Ha, S.; Gross, B.; Walker, S. E. coli MurG: A Paradigm for a Superfamily of Glycosyltransferases. Curr. Drug Targets Infect. Disord. 2001, 1 (2), 201-213. [PubMed Link] [Publisher Link]
Lo, M.-C.; Helm, J. S.; Sarngadharan, G.; Pelczer, I.; Walker, S. A New Structure for the Substrate-Binding Antibiotic Ramoplanin. J. Am. Chem. Soc. 2001, 123 (35), 8640-8641. [PubMed Link] [Publisher Link]
2000
Ha, S.; Walker, D.; Shi, Y.; Walker, S. The 1.9 Å crystal structure of Escherichia coli MurG, a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis. Protein Sci. 2000, 9 (6), 1045-1052. [PubMed Link] [Publisher Link]
Lo, M.-C.; Men, H.; Branstrom, A.; Helm, J.; Yao, N.; Goldman, R.; Walker, S. A New Mechanism of Action Proposed for Ramoplanin. J. Am. Chem. Soc. 2000, 122 (14), 3540-3541. [Publisher Link]
pre-2000
Ha, S.; Chang, E.; Lo, M.-C.; Men, H.; Park, P.; Ge, M.; Walker, S. The Kinetic Characterization of Escherichia coli MurG Using Synthetic Substrate Analogues. J. Am. Chem. Soc. 1999, 121 (37), 8415-8426. [Publisher Link]
Lo, M.-C.; Ha, S.; Pelczer, I.; Pal, S.; Walker, S. The solution structure of the DNA-binding domain of Skn-1. Proc. Natl. Acad. Sci. U. S. A. 1998, 95 (15), 8455-8460. [PubMed Link] [Publisher Link]
Men, H.; Park, P.; Ge, M.; Walker, S. Substrate Synthesis and Activity Assay for MurG. J. Am. Chem. Soc. 1998, 120 (10), 2484-2485. [Publisher Link]
Pal, S.; Lo, M.-C.; Schmidt, D.; Pelczer, I.; Thurber, S.; Walker, S. Skn-1: Evidence for a bipartite recognition helix in DNA binding. Proc. Natl. Acad. Sci. U. S. A. 1997, 94 (11), 5556-5561. [PubMed Link] [Publisher Link]